Name: Anti-CaV3.3 (CACNA1I) Antibody
Brand: Alomone Labs
SKU: ACC-009

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Overview

Cat #: ACC-009

Alternative Name Voltage-dependent T-type calcium channel subunit α_1I

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CNGRMPNIAKDVFTK, corresponding to amino acid residues 1053-1067 of rat Ca_V3.3 (Accession number Q9Z0Y8). Intracellular, between domains II and III.

Accession (Uniprot) Number Q9Z0Y8

Gene ID 56827

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human - 14/15 amino acid residues identical.

RRID AB_2039783.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.75 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, wb

May also work in: ifc*, ip*

Western blot

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
1-2. Anti-Ca_V3.3 (CACNA1I) Antibody (#ACC-009), (1:400).
3-4. Anti-CaV3.3 (CACNA1I) Antibody, preincubated with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009).

Immunohistochemistry

Mouse hindbrain (Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.).

Immunocytochemistry

Human sperm cells (1:50) (Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.).

Scientific background

T-type Ca²⁺ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation.

T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.¹ Three genes encoding T-type Ca²⁺ channels have been cloned and designated as Ca_V3.1 (CACNA1G), Ca_V3.2 (CACNA1H) and Ca_V3.3 (CACNA1I).^1-3

While Ca_V3.1 and Ca_V3.2 are widely expressed in various tissues, Ca_V3.3 is primarily expressed in the central nervous system, where high expression has been described in thalamic neurons.

The Ca²⁺ current generated by the Ca_V3.3 channel displays much slower activation and inactivation compared to the currents produced by Ca_V3.1 and Ca_V3.2, suggesting it might play a different role in neuronal excitability.^1,4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

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Anti-Ca_V3.3 (CACNA1I) Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

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