Name: Anti-CACNB3 Antibody
Brand: Alomone Labs
SKU: ACC-008

Overview

Cat #: ACC-008

Alternative Name Ca_Vβ3, CAB3, CACNLB3, Voltage-dependent L-type calcium channel subunit beta-3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DRNWQRNRPWPKDSY, corresponding to amino acid residues 463-477 of rabbit CACNB3 (Accession P54286). Intracellular, C-terminus.

Accession (Uniprot) Number P54286

Gene ID 100009402

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse, human, Xenopus - identical; bovine - 14/15 amino acid residues identical.

RRID AB_2039787.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Identical for all splice forms.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.3 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, wb

May also work in: ifc*, ip*

Western blot

Western blot analysis of rat brain membranes:
1. Anti-CACNB3 Antibody (#ACC-008), (1:200).
2. Anti-CACNB3 Antibody, preincubated with CACNB3 Blocking Peptide (#BLP-CC008).

Immunohistochemistry

Expression of CACNB3 (Ca_Vβ3) in rat hippocampus
Immunohistochemical staining of rat hippocampus with Anti-CACNB3 Antibody (#ACC-008). A. Ca_Vβ3 (red) appears in neurons (arrows). B. Staining of nerve cells with mouse anti-parvalbumin demonstrates the restriction of Ca_Vβ3 to cell bodies. C. Merged image of panels A and B.

Immunocytochemistry

A7r5 cell line, derived from the rat thoracic aorta (1:400) (Uchida, R. et al. (2003) Eur. J. Pharmacol. 466, 53.).

References

Arikkath, J. and Campbell, K.P. (2003) Curr. Opin. Neurobiol. 13, 298.
Dolphin, A.C. (2003) J. Bioenerg. Biomembr. 35, 599.
Richards, M.W. et al. (2004) Trends Pharmacol. Sci. 25, 626.

Scientific background

The β3 protein is an auxiliary subunit of the Ca_V (voltage-dependent calcium) channel multimer complex. Four β isoforms were identified in mammalian genomes and they share some sequence as well as structure homology.^1-3The structure of Ca_Vβ3 was recently determined (as well as other Ca_Vβ subunits; for review see reference 3). Ca_Vβ subunits are cytoplasmic proteins, that bind the cytoplasmic face of the pore forming Ca_V α1 subunits. These interactions have profound effects on channel trafficking to the plasma membrane as well as on current parameters such as voltage-dependent kinetics and interactions with modulatory proteins.^1,2 Ca_Vβ3 might interact with all the pore-forming subunits that have been tested and in vivo is expressed in smooth muscle, trachea, aorta, lung and brain.¹

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rabbit Ca_Vβ3. Anti-CACNB3 Antibody (#ACC-008) can be used in western blot, immunohistochemistry and immunocytochemistry applications. The antibody has been designed to recognize Ca_Vβ3 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Western blot citations

Transfected HEK 293 cell lysate (1:5000).
Bourdin, B. et al. (2015) J. Biol. Chem. 290, 2854.

Immunocytochemistry citations

A7r5 cell line, derived from the rat thoracic aorta (1:400).
Uchida, R. et al. (2003) Eur. J. Pharmacol. 466, 53.

More product citations

Li, L. et al. (2012) J. Biol. Chem. 287, 6002.
Dilger, E.K. et al. (2011) J. Physiol. 589.4, 919.
Thompson, W.R. et al. (2011) J. Bone Min. Res. 26, 2125.

Specifications

Scientific Background

Specific Control Product

CACNB3 Blocking Peptide (#BLP-CC008) is the original antigen used for immunization during Anti-CACNB3 Antibody (#ACC-008) generation. The blocking peptide binds and ‘blocks’ Anti-CACNB3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

CACNB3 Blocking Peptide (#BLP-CC008)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Anti-CACNB3 Antibody