Overview
Cat #:
ACC-008
Alternative Name CaVβ3, CAB3, CACNLB3, Voltage-dependent L-type calcium channel subunit beta-3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
ImmunogenPeptide (C)DRNWQRNRPWPKDSY, corresponding to amino acid residues 463-477 of rabbit CACNB3 (Accession
P54286). Intracellular, C-terminus.
Accession (Uniprot) Number P54286
Gene ID 100009402
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, human, Xenopus - identical; bovine - 14/15 amino acid residues identical.
RRID AB_2039787.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Specificity Identical for all splice forms.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.3 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
ImmunocytochemistryA7r5 cell line, derived from the rat thoracic aorta (1:400) (
Uchida, R. et al. (2003)
Eur. J. Pharmacol. 466, 53.).
Scientific background The β3 protein is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. Four β isoforms were identified in mammalian genomes and they share some sequence as well as structure homology.1-3 The structure of CaVβ3 was recently determined (as well as other CaVβ subunits; for review see reference 3). CaVβ subunits are cytoplasmic proteins, that bind the cytoplasmic face of the pore forming CaV α1 subunits. These interactions have profound effects on channel trafficking to the plasma membrane as well as on current parameters such as voltage-dependent kinetics and interactions with modulatory proteins.1,2 CaVβ3 might interact with all the pore-forming subunits that have been tested and in vivo is expressed in smooth muscle, trachea, aorta, lung and brain.1
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Western blot analysis of rat brain membranes:1. Anti-CACNB3 Antibody (#ACC-008), (1:200).
