Overview
Alternative Name Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)DNFDYLTRDWSILGPHHLD, corresponding to amino acid residues 1506-1524 of rat CaV1.2 (Accession P22002). Intracellular, C-terminus region.
Accession (Uniprot) Number P22002
Gene ID 24239
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology CaV1 family (α1C, α1D, α1F, α1S) from all known species - identical; CaV2 family (α1A, α1B, α1E) from all known species - 17/19 amino acid residues identical.
RRID AB_2039762.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.3 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, ip, wb
May also work in: ic*, ifc*
Western blot
- Rat brain membranes (1:200).
Western blot analysis of rat brain membranes:1. Anti-CaVpan α1 Antibody (#ACC-004), (1:200).
2. Anti-CaVpan α1 Antibody, preincubated with Pan CaV α 1 Blocking Peptide (#BLP-CC004).
Immunoprecipitation
- Rat basilar artery lysates (Murphy, K. et al. (2003) Mol. Pharmacol. 64, 640.).
Immunohistochemistry
- Human placenta (1:300) (Bernucci, L. et al. (2006) Placenta 27, 1082.).
Scientific background
Ca2+ ions regulate many cellular processes such as secretion, proliferation, and apoptosis and they are also cofactors of many proteins.1,2 In neurons, Ca2+ entry through voltage-dependent Ca2+ (CaV) channels triggers neurotransmitter release. Fast synaptic transmission in the mammalian central nervous system (CNS) is mediated by several high voltage-dependent Ca2+ channels.3
Five types of Ca2+ channels are expressed in the CNS: the L-type (CaV1), N-type (CaV2.2), P/Q-type (CaV2.1), R-type (CaV2.3), and the T-type (CaV3).4 Each CaV channel is a multimeric protein composed of a pore forming α1 subunit and the auxiliary β (CaVβ), α2δ and γ subunits. There are four known CaVβ subunits, in addition to four α2δ subunits and eight γ subunits.4
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
Citations
