Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-041), and immunohistochemical staining (labeled antibody).
Applications: ih
May also work in: ic*, lci*
Immunohistochemistry
Rat brain sections.
Expression of CD56 in rat brain
Immunohistochemical staining of rat olfactory bulb free-floating frozen sections using Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody (#ANR-041-AG), (1:60). CD56 immunoreactivity (green) appears in glomerular structures (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
Scientific background
Neural cell adhesion molecule 1 (NCAM-1) is a member of a large family of cell-surface glycoproteins and plays a major role during development and controls various functions in the nervous system such as cell migration, neurite growth, axonal guidance, and synaptic plasticity1. NCAM-1 mediates adhesion between cells through homophilic bonds formed between its extracellular domains, which comprise five tandem Ig domains and two juxtamembrane fibronectin type III (Fn III) domains2. There are three major protein isoforms of NCAM-1: 180 kd, 140 kd, and 120 kd. The 180-kd and 140-kd isoforms of NCAM-1 are transmembrane proteins, whereas the 120-kd isoform is linked to the plasma membrane via a glycosyl phosphatidyl-inositol (GPI) lipid anchor3.
NCAM-1 is widely expressed in the central nervous system, skeletal muscle and natural killer cells4.
Several lines of evidence have supported the idea that dysregulation of NCAM-1 isoforms in the brain might be involved in the pathophysiology of neuropsychiatric disorders, particularly bipolar affective disorder5.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Expression of CD56 in rat brainImmunohistochemical staining of rat olfactory bulb free-floating frozen sections using Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody (#ANR-041-AG), (1:60). CD56 immunoreactivity (green) appears in glomerular structures (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).