Name: Anti-CD73 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: ANT-066-F

Overview

Cat #: ANT-066-F

Alternative Name Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E, ENT

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73 (Accession Q61503). Extracellular, N-terminus.

Accession (Uniprot) Number Q61503

Gene ID 23959

Homology Human, rat - identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANT-066), and direct flow cytometry (labeled antibody).

Applications: fc

Direct flow cytometry

Cell surface detection of CD73 by direct flow cytometry in live intact human Burkitt's lymphoma Daudi cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-CD73 (extracellular)-FITC Antibody (#ANT-066-F) (5µg).
Cell surface detection of CD73 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-CD73 (extracellular)-FITC Antibody (#ANT-066-F) (5µg).

References

Chen, S. et al. (2019) Nat. Commun. 10, 150.
Kordaß, T. et al. (2018) Front. Immunol. 9, 813.
Wang, R. et al. (2017) Oncotarget 8, 57327.

Scientific background

CD73 (ecto-5′-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.

CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses^1,2.

CD73 primarily detected in cancer cells and in immune cells such as CD4⁺Foxp3⁺ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor².

Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-CD73 (extracellular) Antibody (#ANT-066) is a highly specific antibody directed against an extracellular epitope of the mouse NT5E. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize NT5E from mouse, rat and human samples.

Anti-CD73 (extracellular)-FITC Antibody (#ANT-066-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize NT5E from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 09/06/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Reagent Rewards

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Navigation

Order Summary

Anti-CD73 (extracellular)-FITC Antibody