Overview
- Peptide CHQKIDDLFSGK, corresponding to amino acid residues 190 - 201 of human CD81 (Accession P60033). Extracellular, 2nd loop.
CD81 (extracellular) Blocking Peptide (#BLP-NR211)
- Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and mouse liver lysate (lanes 3 and 6):1-3. Anti-CD81 (extracellular) Antibody (#ANR-211), (1:400).
4-6. Anti-CD81 (extracellular) Antibody, preincubated with CD81 (extracellular) Blocking Peptide (BLP-NR211). - Western blot analysis of human PC3 prostate adenocarcinoma cell line lysates (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):1-2. Anti-CD81 (extracellular) Antibody (#ANR-211), (1:200).
3-4. Anti-CD81 (extracellular) Antibody, preincubated with CD81 (extracellular) Blocking Peptide (BLP-NR211). - Western blot analysis of mouse BV-2 cell line lysate (lane 1) and BV2-derived purified exosomes (lane 2)50µg of cells and purified exosomes lysates were analyzed using Anti-CD81 (extracellular) Antibody (#ANR-211), (1:200).
- Western blot analysis of human U-87 MG glioblastoma cell line lysate (lane 1) and U-87 MG-derived purified exosomes (lane 2)50µg of cells and purified exosomes lysates were analyzed using Anti-CD81 (extracellular) Antibody (#ANR-211), (1:400).
- Expression of CD81 in rat medial habenula.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CD81 (extracellular) Antibody (#ANR-211), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CD81 immunoreactivity (green) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with CD81 (extracellular) Blocking Peptide (BLP-NR211), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Cell surface detection of CD81 by indirect flow cytometry in live intact human Raji lymphoblast B-cell line:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-CD81 (extracellular) Antibody (#ANR-211), (5μg) + goat-anti-rabbit-FITC. - Cell surface detection of CD81 by indirect flow cytometry in live intact mouse BV-2 microglia cell line:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-CD81 (extracellular) Antibody (#ANR-211), (2.5μg) + goat-anti-rabbit-FITC. - Cell surface detection of CD81 by indirect flow cytometry in BV-2- derived exosomes:___ Exosomes only.
___ Exosomes + goat-anti-rabbit-FITC.
___ Exosomes + Anti-CD81 (extracellular) Antibody (#ANR-211), (5μg) + goat-anti-rabbit-FITC.
- Bailly, C. and Thuru, X. (2023) Cancers. 15,7.
- Zimmerman, B. et al. (2016) Cell. 167,4.
- Shoham, T. et al. (2006) Mol. Cell. Biol. 26,4.
Cluster of differentiation 81, CD81, also known as Tetraspanin-28, Tspan28, and target of the antiproliferative antibody 1, TAPA-1, is a transmembrane (TM) protein, member of the tetraspanin family, and plays a role in intercellular interactions and cellular trafficking.1
CD81 is composed of 236 amino acids, four TM regions, intracellular N- and C-termini, small (EC1) and large (EC2) extracellular loops, a short intracellular loop between TM2 and TM3, and is characterized by a bound cholesterol molecule in an intramembrane pocket between the two pairs of TM helices capped by the EC2 domain. The binding of cholesterol alters the conformation of the protein, a process which is exploited by certain viruses to enter cells. CD81 serves as a co-receptor for several viruses, including hepatitis C (HCV), human immunodeficiency virus type 1 (HIV-1), herpes simplex virus 1 (HSV-1), influenza A (IAV), and Chikungunya, as well as certain bacteria and parasites. CD81 can interact with other TM proteins to form clusters and higher-order protein complexes.1,2
CD81 is highly conserved in mammals, expressed on all primary B-lineage cells from early B-cell development stages, and associates with CD19 on the cell surface. CD81 engagement on mature human B-cells induces an antiproliferative effect.3
CD81 is involved in neurotransmission, cell signaling, cell proliferation, and protein trafficking, as well as the immune, reproductive, genitourinary, and auditory systems. CD81 is also implicated in oncology, viral infection, and inflammation.1,2
Application key:
Species reactivity key:
Anti-CD81 (extracellular) Antibody (#ANR-211) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD81 from mouse, rat and human samples.