Name: Anti-CD81 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANR-211

Back to product page SDS

Overview

Cat #: ANR-211

Alternative Name Tetraspanin-28, TSPAN28, Target of the Antiproliferative Antibody 1, TAPA-1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CHQKIDDLFSGK, corresponding to amino acid residues 190 - 201 of human CD81 (Accession P60033). Extracellular, 2^nd loop.

Accession (Uniprot) Number P60033

Gene ID 975

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat – 11 out of 12 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 2 µg peptide per 1 µg antibody
CD81 (extracellular) Blocking Peptide (#BLP-NR211)

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and mouse liver lysate (lanes 3 and 6):
1-3. Anti-CD81 (extracellular) Antibody (#ANR-211), (1:400).
4-6. Anti-CD81 (extracellular) Antibody, preincubated with CD81 (extracellular) Blocking Peptide (BLP-NR211).
Western blot analysis of human PC3 prostate adenocarcinoma cell line lysates (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):
1-2. Anti-CD81 (extracellular) Antibody (#ANR-211), (1:200).
3-4. Anti-CD81 (extracellular) Antibody, preincubated with CD81 (extracellular) Blocking Peptide (BLP-NR211).
Western blot analysis of mouse BV-2 cell line lysate (lane 1) and BV2-derived purified exosomes (lane 2)

50µg of cells and purified exosomes lysates were analyzed using Anti-CD81 (extracellular) Antibody (#ANR-211), (1:200).
Western blot analysis of human U-87 MG glioblastoma cell line lysate (lane 1) and U-87 MG-derived purified exosomes (lane 2)
50µg of cells and purified exosomes lysates were analyzed using Anti-CD81 (extracellular) Antibody (#ANR-211), (1:400).

Indirect flow cytometry

Cell surface detection of CD81 by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-CD81 (extracellular) Antibody (#ANR-211), (2.5μg) + goat-anti-rabbit-FITC.
Cell surface detection of CD81 by indirect flow cytometry in BV-2- derived exosomes:
^___ Exosomes only.
^___ Exosomes + goat-anti-rabbit-FITC.
^___ Exosomes + Anti-CD81 (extracellular) Antibody (#ANR-211), (5μg) + goat-anti-rabbit-FITC.

Scientific background

Cluster of differentiation 81, CD81, also known as Tetraspanin-28, Tspan28, and target of the antiproliferative antibody 1, TAPA-1, is a transmembrane (TM) protein, member of the tetraspanin family, and plays a role in intercellular interactions and cellular trafficking.¹

CD81 is composed of 236 amino acids, four TM regions, intracellular N- and C-termini, small (EC1) and large (EC2) extracellular loops, a short intracellular loop between TM2 and TM3, and is characterized by a bound cholesterol molecule in an intramembrane pocket between the two pairs of TM helices capped by the EC2 domain. The binding of cholesterol alters the conformation of the protein, a process which is exploited by certain viruses to enter cells. CD81 serves as a co-receptor for several viruses, including hepatitis C (HCV), human immunodeficiency virus type 1 (HIV-1), herpes simplex virus 1 (HSV-1), influenza A (IAV), and Chikungunya, as well as certain bacteria and parasites. CD81 can interact with other TM proteins to form clusters and higher-order protein complexes.^1,2

CD81 is highly conserved in mammals, expressed on all primary B-lineage cells from early B-cell development stages, and associates with CD19 on the cell surface. CD81 engagement on mature human B-cells induces an antiproliferative effect.³

CD81 is involved in neurotransmission, cell signaling, cell proliferation, and protein trafficking, as well as the immune, reproductive, genitourinary, and auditory systems. CD81 is also implicated in oncology, viral infection, and inflammation.^1,2

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Navigation

Order Summary

Certificate of Analysis

Anti-CD81 (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

Need Help With This Product?