Name: Anti-CD81 (extracellular)-APC Antibody
Brand: Alomone Labs
SKU: ANR-211-APC

Overview

Cat #: ANR-211-APC

Alternative Name Tetraspanin-28, TSPAN28, Target of the Antiproliferative Antibody 1, TAPA-1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CHQKIDDLFSGK, corresponding to amino acid residues 190 - 201 of human CD81 (Accession P60033). Extracellular, 2^nd loop.

Accession (Uniprot) Number P60033

Gene ID 975

Homology Mouse, rat – 11 out of 12 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Allophycocyanin (APC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-211), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of CD81 by direct flow cytometry in live intact mouse BV-2 microglia cell line:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-APC (#RIC-001-APC).
^___ Cells + Anti-CD81 (extracellular)-APC Antibody (#ANR-211-APC), (2.5µg).
Cell surface detection of CD81 by direct flow cytometry in live intact human U-87 MG glioblastoma cell line:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-APC (#RIC-001-APC).
^___ Cells + Anti-CD81 (extracellular)-APC Antibody (#ANR-211-APC), (2.5µg).

References

Bailly, C. and Thuru, X. (2023) Cancers. 15,7.
Zimmerman, B. et al. (2016) Cell. 167,4.
Shoham, T. et al. (2006) Mol. Cell. Biol. 26,4.

Scientific background

Cluster of differentiation 81, CD81, also known as Tetraspanin-28, Tspan28, and target of the antiproliferative antibody 1, TAPA-1, is a transmembrane (TM) protein, member of the tetraspanin family, and plays a role in intercellular interactions and cellular trafficking.¹

CD81 is composed of 236 amino acids, four TM regions, intracellular N- and C-termini, small (EC1) and large (EC2) extracellular loops, a short intracellular loop between TM2 and TM3, and is characterized by a bound cholesterol molecule in an intramembrane pocket between the two pairs of TM helices capped by the EC2 domain. The binding of cholesterol alters the conformation of the protein, a process which is exploited by certain viruses to enter cells. CD81 serves as a co-receptor for several viruses, including hepatitis C (HCV), human immunodeficiency virus type 1 (HIV-1), herpes simplex virus 1 (HSV-1), influenza A (IAV), and Chikungunya, as well as certain bacteria and parasites. CD81 can interact with other TM proteins to form clusters and higher-order protein complexes.^1,2

CD81 is highly conserved in mammals, expressed on all primary B-lineage cells from early B-cell development stages, and associates with CD19 on the cell surface. CD81 engagement on mature human B-cells induces an antiproliferative effect.³

CD81 is involved in neurotransmission, cell signaling, cell proliferation, and protein trafficking, as well as the immune, reproductive, genitourinary, and auditory systems. CD81 is also implicated in oncology, viral infection, and inflammation.^1,2

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-CD81 (extracellular) Antibody (#ANR-211) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CD81 from mouse, rat and human samples.

Anti-CD81 (extracellular)-APC Antibody (#ANR-211-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 28/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-APC (#RIC-001-APC), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to Allophycocyanin (APC).

Rabbit IgG Isotype Control-APC (#RIC-001-APC)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-CD81 (extracellular)-APC Antibody