Name: Anti-CHERP Antibody
Brand: Alomone Labs
SKU: ACC-330

Overview

Cat #: ACC-330

Alternative Name Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)KLEDHEYKPLDPKDIR, corresponding to amino acid residues 669-684 of mouse CHERP (Accession Q8CGZ0). Cytoplasm.

Accession (Uniprot) Number Q8CGZ0

Gene ID 27967

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, human - identical.

RRID AB_2756560.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse C2C12 muscle myoblast cell line (lanes 3 and 6) lysates:
1-3. Anti-CHERP Antibody (#ACC-330), (1:1500).
4-6. Anti-CHERP Antibody, preincubated with CHERP Blocking Peptide (#BLP-CC330).

Immunohistochemistry

Expression of CHERP in mouse brain
Immunohistochemical staining of mouse brain sections using Anti-CHERP Antibody (#ACC-330). A. In mouse hippocampal CA3 region, CHERP immunoreactivity (green) appears in neurons (arrows) of CA3 pyramidal layer (P). B. In parietal cortex, CHERP staining (green) is detected in neurons (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).

References

Lin-Moshier, Y. et al. (2013) J. Biol. Chem. 288, 355.
Sasaki-Osugi, K. et al. (2013) J. Biol. Chem. 288, 33361.
Ryan, T. et al. (2011) J. Biol. Chem. 286, 17060.

Scientific background

CHERP (calcium homeostasis endoplasmic reticulum protein) is a ubiquitously expressed integral sarco/endoplasmic reticulum (SR/ER) membrane protein. CHERP protein consist of 916 amino acid in humans and its structure containing a Pro-rich region and a segment of Arg-Ser dipeptide repeats (an RS domain) near the C terminus, and an RNA recognition motifs (RRMs)^1-3.

CHERP acts as a regulator of both major families of endoplasmic reticulum Ca²⁺ channels, inositol 1,4,5-trisphosphate receptors (IP₃Rs) and ryanodine receptors (RyRs). CHERP is responsible for the regulation of intracellular Ca²⁺ mobilization in human cells and is involved in neuroblastoma cell proliferation, apoptosis and tumorigenicity^1,2.

CHERP co-localizes with IP₃ receptors throughout the cytoplasmic and perinuclear regions in human erythroleukemia cells and in Jurkat cells. CHERP is also detected in the nucleoplasm with prominent accumulation at nuclear speckles^1,2. CHERP co-localizes with endogenous ryanodine receptor type 1 (RyR1) in the sarcoplasmic reticulum of rat soleus muscle. Studies of CHERP knockdown reveal decreased intracellular Ca²⁺ mobilization, cell growth and proliferation^2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-CHERP Antibody (#ACC-330) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CHERP from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

CHERP Blocking Peptide (#BLP-CC330) is the original antigen used for immunization during Anti-CHERP Antibody (#ACC-330) generation. The blocking peptide binds and ‘blocks’ Anti-CHERP primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

CHERP Blocking Peptide (#BLP-CC330)

Shipping and Ordering Information

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Anti-CHERP Antibody