Name: Anti-CHERP-ATTO Fluor-594 Antibody
Brand: Alomone Labs
SKU: ACC-330-AR

Overview

Cat #: ACC-330-AR

Alternative Name Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KLEDHEYKPLDPKDIR, corresponding to amino acid residues 669-684 of mouse CHERP (Accession Q8CGZ0). Cytoplasm.

Accession (Uniprot) Number Q8CGZ0

Gene ID 27967

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, human - identical.

RRID AB_2756561.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 – 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody #ACC-330), and immunohistochemical staining (labeled antibody).

Applications: if, ih

May also work in: ic*

Immunohistochemistry

Expression of CHERP in mouse brain
Immunohistochemical staining of mouse cortex and hippocampus using Anti-CHERP-ATTO Fluor-594 Antibody (#ACC-330-AR). A. CHERP staining (red) in cortex appears in in cortical neurons (arrows). B. In hippocampus, CHERP staining (red) appears in neurons of pyramidal layer (P) in CA3 region. Nuclei are stained using DAPI (blue).

References

Lin-Moshier, Y. et al. (2013) J. Biol. Chem. 288, 355.
Sasaki-Osugi, K. et al. (2013) J. Biol. Chem. 288, 33361.
Ryan, T. et al. (2011) J. Biol. Chem. 286, 17060.

Scientific background

CHERP (calcium homeostasis endoplasmic reticulum protein) is a ubiquitously expressed integral sarco/endoplasmic reticulum (SR/ER) membrane protein. CHERP protein consist of 916 amino acid in humans and its structure containing a Pro-rich region and a segment of Arg-Ser dipeptide repeats (an RS domain) near the C terminus, and an RNA recognition motifs (RRMs)^1-3.

CHERP acts as a regulator of both major families of endoplasmic reticulum Ca²⁺ channels, inositol 1,4,5-trisphosphate receptors (IP₃Rs) and ryanodine receptors (RyRs). CHERP is responsible for the regulation of intracellular Ca²⁺ mobilization in human cells and is involved in neuroblastoma cell proliferation, apoptosis and tumorigenicity^1,2.

CHERP co-localizes with IP₃ receptors throughout the cytoplasmic and perinuclear regions in human erythroleukemia cells and in Jurkat cells. CHERP is also detected in the nucleoplasm with prominent accumulation at nuclear speckles^1,2. CHERP co-localizes with endogenous ryanodine receptor type 1 (RyR1) in the sarcoplasmic reticulum of rat soleus muscle. Studies of CHERP knockdown reveal decreased intracellular Ca²⁺ mobilization, cell growth and proliferation^2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-CHERP Antibody (#ACC-330) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize CHERP from human, rat, and mouse samples.

Anti-CHERP-ATTO Fluor-594 Antibody (#ACC-330-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CHERP-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 13/02/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

CHERP Blocking Peptide (#BLP-CC330) is the original antigen used for immunization during Anti-CHERP Antibody (#ACC-330) generation. The blocking peptide binds and ‘blocks’ Anti-CHERP primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

CHERP Blocking Peptide (#BLP-CC330)

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Anti-CHERP-ATTO Fluor-594 Antibody