Overview
- Peptide (C)RNKENHKPTESSLDEK, corresponding to amino acid residues 87 - 102 of mouse CLEC7A (Accession Q6QLQ4). Extracellular, C-terminus.
Not recommended for human samples.
Cell surface detection of CLEC7A/Dectin-1 by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-CLEC7A/Dectin-1 (extracellular)-FITC Antibody (#ALR-062-F), (2.5µg).
Cell surface detection of CLEC7A/Dectin-1 by direct flow cytometry in live intact mouse BV-2 microglia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-CLEC7A/Dectin-1 (extracellular)-FITC Antibody (#ALR-062-F), (2.5µg).
- Goodridge, H.S., et al., (2011) Nature, 472(7344): p. 471-5.
- Kalia, N., J. Singh, and M. Kaur, (2021) Immunobiology, 226(2): p. 152071.
- Brown, G.D., et al.,(2003) J Exp Med, 197(9): p. 1119-24.
- McDonald, J.U., et al., (2012) PLoS One, 7(9): p. e45781.
- Taylor, P.R., et al., (2002) J Immunol, 169(7): p. 3876-82.
- Kimura, Y., et al., (2014) J Biol Chem, 289(45): p. 31565-75.
- Willment, J.A., et al., (2003) J Immunol, 171(9): p. 4569-73.
- Chen, K.H., et al., (2016) Int J Biochem Cell Biol, 74: p. 135-44.
- Pamuk, O.N., et al., (2015) Exp Rheumatol, 33(4 Suppl 91): p. S15-22.
Dectin-1, encoded by CLEC7A, is a small transmembrane receptor that is a member of the C-type lectin family of pattern-recognition receptors (PRRs)1,2. As this receptor specifically recognizes and binds to β-glucans polysaccharides derived from the cell walls of fungi and yeast, it has been characterized for its role in antifungal immunity3.
Dectin-1 is predominately expressed on macrophages, dendritic cells, and neutrophils4,5. Upon ligation of β-glucan, Dectin-1 recruits the adaptor protein CARD9 to phosphorylate spleen tyrosine kinase (Syk) which in turn initiates a signaling cascade which induces phagocytosis and leads to the activation of nuclear factor-κB (NF-κB) and MAPK6. The Dectin-1 Syk mediated pathway has been demonstrated to be activated and involved in the alternative activation of macrophages as Dectin-1 was upregulated in vitro within 4 hours when stimulated with IL-4 and IL-13, cytokines which induce alternative activation of macrophages7. Moreover, the activated Syk pathway has also been implicated in renal interstitial fibrotic disease where it was demonstrated that suppressing Syk in an experimental mouse model of renal fibrosis inhibits the progression of kidney fibrosis, shown by a reduction in mRNA expression of Arginase-1 (murine M2 macrophage marker)8.
While the Dectin-1 pathway has been shown to be involved in the bleomycin-induced model of skin fibrosis, Dectin-1 signaling and receptor expression has yet to be investigated in the context of pulmonary fibrosis9.
Anti-CLEC7A/Dectin-1 (extracellular) Antibody (#ALR-062) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize CLEC7A from mouse and rat samples. The antibody will not recognize CLEC7A from human samples.
Anti-CLEC7A/Dectin-1 (extracellular)-FITC Antibody (#ALR-062-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize CLEC7A from mouse and rat samples. The antibody will not recognize CLEC7A from human samples.
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