Name: Anti-CNIH2/3 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGC-047

Overview

Cat #: AGC-047

Alternative Name Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide RARERLKNIERIC, corresponding to amino acid residues 49-61 of rat CNIH2 (Accession Q5BJU5) and rat CNIH3 (Accession D0Q0Y7). Extracellular loop.

Accession (Uniprot) Number Q5BJU5 and D0Q0Y7

Gene ID 361705 and 690252

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology CNIH2 = Mouse, human identical.
CNIH3 = Rat, mouse, human – 11/13 amino acid residues identical.

RRID AB_2756602.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity This antibody will recognize both CNIH2 and CNIH3, but not the related proteins CNIH1 and CNIH4.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*, lci*

Western blot

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
1,2. Anti-CNIH2/3 (extracellular) Antibody (#AGC-047), (1:200).
3,4. Anti-CNIH2/3 (extracellular) Antibody, preincubated with CNIH2/3 (extracellular) Blocking Peptide (#BLP-GC047).

References

Erlenhardt, N. et al. (2016) Cell Rep. 14, 782.
Shanks, N.F. et al. (2014) J. Neurosci. 34, 12104.
Haering, S.C. et al. (2014) Membranes (Basel) 4, 469.
Herring, B.E. et al. (2013) Neuron 77,1083.

Scientific background

Cornichon homologs (CNIHs) are a family of AMPA-type glutamate receptor (AMPAR) auxiliary subunits that modulate AMPAR ion channel function and trafficking. Cornichon 2 and 3 (CNIH2/3) are responsible for controlling the export of AMPARs from the endoplasmic reticulum and associate with synaptic AMPARs to modulate channel kinetics^1,2.

CNIHs are small proteins with three transmembrane domains that directly binds to AMPARs. Overexpression CNIHs and AMPA receptors causes their localization to the Golgi apparatus³.

CNIH and TARP both compete for the binding to AMPAR subunits GluA2, GluA3, and GluA4 subunits suggesting both proteins bind to the same site. CNIH2/3 binding to AMPARs is dependent on AMPAR subunit composition and TARPs^3,4.

CNIH2/3 knock out mice shows alterations in synaptic content of GluA1-containing AMPARs, resulting in a reduction in AMPAR-mediated transmission and long-term potentiation².

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-CNIH2/3 (extracellular) Antibody (#AGC-047) is a highly specific antibody directed against a conserved sequence of the rat CNIH2 and CNIH3 proteins. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the proteins in living cells. It has been designed to recognize CNIH2 and CNIH3 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

CNIH2/3 (extracellular) Blocking Peptide (#BLP-GC047) is the original antigen used for immunization during Anti-CNIH2/3 (extracellular) Antibody (#AGC-047) generation. The blocking peptide binds and ‘blocks’ Anti-CNIH2/3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

CNIH2/3 (extracellular) Blocking Peptide (#BLP-GC047)

Shipping and Ordering Information

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Anti-CNIH2/3 (extracellular) Antibody