Overview
Cat #:
AGC-047
Alternative Name Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
ImmunogenPeptide RARERLKNIERIC, corresponding to amino acid residues 49-61 of rat CNIH2 (Accession
Q5BJU5) and rat CNIH3 (Accession
D0Q0Y7). Extracellular loop.
Accession (Uniprot) Number Q5BJU5 and D0Q0Y7
Gene ID 361705 and 690252
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology CNIH2 = Mouse, human identical.
CNIH3 = Rat, mouse, human – 11/13 amino acid residues identical.
RRID AB_2756602.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Specificity This antibody will recognize both CNIH2 and CNIH3, but not the related proteins CNIH1 and CNIH4.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Scientific background Cornichon homologs (CNIHs) are a family of AMPA-type glutamate receptor (AMPAR) auxiliary subunits that modulate AMPAR ion channel function and trafficking. Cornichon 2 and 3 (CNIH2/3) are responsible for controlling the export of AMPARs from the endoplasmic reticulum and associate with synaptic AMPARs to modulate channel kinetics1,2.
CNIHs are small proteins with three transmembrane domains that directly binds to AMPARs. Overexpression CNIHs and AMPA receptors causes their localization to the Golgi apparatus3.
CNIH and TARP both compete for the binding to AMPAR subunits GluA2, GluA3, and GluA4 subunits suggesting both proteins bind to the same site. CNIH2/3 binding to AMPARs is dependent on AMPAR subunit composition and TARPs3,4.
CNIH2/3 knock out mice shows alterations in synaptic content of GluA1-containing AMPARs, resulting in a reduction in AMPAR-mediated transmission and long-term potentiation2.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-CNIH2/3 (extracellular) Antibody (#AGC-047), (1:200).
