Overview
- Peptide (C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF (Accession P26441). C-terminus.
Western blot analysis using Anti-CNTF Antibody on:1. Recombinant human CNTF protein (#C-240), 20ng.
2. Recombinant human Cardiotrophin-1 protein (#C-200), 20ng.
3. Recombinant human LIF protein (#L-200), 20ng.
4. mIL-6, 20 ng.
5. Recombinant human CNTF protein (#C-240), 20ng.
Lanes 1-4. Anti-CNTF Antibody (#ANT-031), (1:200).
Lane 5. Anti-CNTF Antibody, preincubated with CNTF Blocking Peptide (#BLP-NT031).
Antibody specificity is demonstrated in that it specifically recognizes CNTF while it does not reacts with the related cytokines Cardiotrophin-1, LIF and IL-6.
Expression of Ciliary neurotrophic factor (CNTF) in rat hippoccampusImmunohistochemical staining of rat hippoccampus using Anti-CNTF Antibody (#ANT-031), (1:200). A. CNTF staining (green) reveals astrocyte-profiles (arrows) in the hilus (H) of the dentate gyrus. B. Staining the same section with the astrocyte marker, anti-glial fibrillary acidic protein (red), visualizes astrocytes in the hilus. C. Merge of the two images shows that CNTF is indeed expressed in astrocytes.
Expression of CNTF in rat C6 glioma cellsImmunocytochemical staining of fixed and permeabilized rat C6 glioma cells. A. Cells were stained with Anti-CNTF Antibody (#ANT-031), (1:200), followed by goat anti-rabbit-DyLight-594 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.
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CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR)5.
CNTF can support the survival of many different cell populations within the PNS and the CNS6. in vitro, CNTF promotes proliferation and neuronal specifications in hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons7, induces sprouting of cholinergic motor neurons8 and delays neural degeneration in genetic models of motor neuron disease9. In addition, it is involved in the development stage of astrocytes and oligodendocytes10.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CNTF. Anti-CNTF Antibody (#ANT-031) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CNTF from human, rat and mouse samples. Anti-CNTF Antibody specifically recognizes CNTF and not related cytokines.
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