Alternative Name Complexin 1, Complexin I, Synaphin-2, Complexin 2, Complexin II, Synaphin-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
Peptide (C)KDMGKMLGGDEEKD, corresponding to amino acid residues 14-27 of rat Complexin-1 (Accession P63041) and rat Complexin-2 (Accession P84087). Intracellular, N-terminus.
Accession (Uniprot) Number P63041, P84087
Gene ID 64832, 116657
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Complexin 1: Mouse, human – identical. Complexin 2: Rat, mouse, human – 13/14 amino acid residues identical.
Specificity The antibody will recognize both Complexin-1 and Complexin-2.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
Mouse and rat brain lysates, human SH-SY5Y neuroblastoma cell lysate (1:200-1:1000).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
Complexins (1 through 4) are small cytosolic proteins that bind tightly to a complex of SNARE proteins to regulate synaptic vesicle membrane fusion via its interaction with synaptotagmin. Complexins activate the quick release of neurotransmitters, triggered by calcium release. They also regulate the “spontaneous release” of neurotransmitters1-3.
Complexins are evolutionarily conserved in mammals, Drosophila, and Caenorhabditis elegans1,2. They are divided into four structural domains: flexible N- and C-terminal domains, an accessory domain, and a central α-helical domain. The N-terminal domain plays a role in activation of fast synchronous release in murine neurons and in isolated chromaffin cells. The C-terminal domain binds to phospholipids and is important for regulating spontaneous release in neuronal cultures and suppressing Ca2+-independent fusion in vitro. The accessory domain is necessary for regulating spontaneous release and the central domain is required for all functions1,2.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-CPLX1/2 Antibody (#ANR-011), (1:200).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-CPLX1/2 Antibody (#ANR-011), (1:200).
