Overview
- Peptide CNVNTHEKVKTALN, corresponding to amino acid residues 127 - 140 of human Calcitonin Receptor-Like Receptor (Accession Q16602). Extracellular, N-terminus.
- Cell surface detection of CRLR/CALCRL by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-CRLR/CALCRL (extracellular)-FITC Antibody (#ACR-060-F), (2.5µg). - Cell surface detection of CRLR/CALCRL by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-CRLR/CALCRL (extracellular)-FITC Antibody (#ACR-060-F), (5µg).
- Singh, Y. et al. (2017) CNS Neurosci. Ther. 23, 457.
- Conner, A.C. et al. (2002) Biochem. Soc. Trans. 30, 451.
- Holland, P.R. and Goadsby, P.J. (2018) Neurotherapeutics 15, 304.
Calcitonin Receptor-Like Receptor 1 (CRLR or CGRPR) is a G protein-coupled receptor that binds the peptide hormone calcitonin and is involved in maintenance of calcium homeostasis, particularly with respect to bone formation and metabolism1.
When engaged with its substrate, calcitonin gene related peptide (CGRP), CGRPR activates cAMP-dependent pkA and PI3 kinase, which eventually leads to complex inhibitory as well as facilitator actions on Neuronal Nicotinic Acetylcholine Receptor1.
CGRP receptor is a heterodimer composed of two proteins: a 7‐transmembrane domain type 2 G-protein coupled receptor called calcitonin-receptor-like receptor (CRLR), and receptor-activity-modifying protein 1 (RAMP1), a single-membrane-pass protein. RAMP1 serves as a transporter for CRLR to the cell surface and its absence leads to failure of binding with CRLR2.
CGRP and CGRPR are ubiquitously expressed and their interaction is related to various diseases and health states such as migraines3 and Alzheimer's disease1.
Application key:
Species reactivity key:
Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize CALCRL from human, mouse and rat samples.
Anti-CRLR/CALCRL (extracellular)-FITC Antibody (#ACR-060-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging