Name: Anti-CRLR/CALCRL (extracellular)-PE Antibody
Brand: Alomone Labs
SKU: ACR-060-PE

Overview

Cat #: ACR-060-PE

Alternative Name Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CNVNTHEKVKTALN, corresponding to amino acid residues 127 - 140 of human Calcitonin Receptor-Like Receptor (Accession Q16602). Extracellular, N-terminus.

Accession (Uniprot) Number Q16602

Gene ID 10203

Homology Mouse, rat – 12 out of 14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label R-Phycoerythrin (R-PE)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACR-060), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of CALCRL by direct flow cytometry in live intact human KU812 basophilic leukemia cell line:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-PE.
^___ Cells + Anti-CRLR/CALCRL (extracellular)-PE Antibody (#ACR-060-PE), (2.5µg).
Cell surface detection of CALCRL by direct flow cytometry in live intact mouse J774 macrophage cell line:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-PE (#RIC-001-PE).
^___ Cells + Anti-CRLR/CALCRL (extracellular)-PE Antibody (#ACR-060-PE), (5µg).

References

Singh, Y. et al. (2017) CNS Neurosci. Ther. 23, 457.
Conner, A.C. et al. (2002) Biochem. Soc. Trans. 30, 451.
Holland, P.R. and Goadsby, P.J.(2018) Neurotherapeutics 15, 304.

Scientific background

Calcitonin Receptor-Like Receptor 1 (CRLR or CGRPR) is a G protein-coupled receptor that binds the peptide hormone calcitonin and is involved in maintenance of calcium homeostasis, particularly with respect to bone formation and metabolism¹.

When engaged with its substrate, calcitonin gene related peptide (CGRP), CGRPR activates cAMP-dependent pkA and PI3 kinase, which eventually leads to complex inhibitory as well as facilitator actions on Neuronal Nicotinic Acetylcholine Receptor¹.

CGRP receptor is a heterodimer composed of two proteins: a 7‐transmembrane domain type 2 G-protein coupled receptor called calcitonin-receptor-like receptor (CRLR), and receptor-activity-modifying protein 1 (RAMP1), a single-membrane-pass protein. RAMP1 serves as a transporter for CRLR to the cell surface and its absence leads to failure of binding with CRLR².

CGRP and CGRPR are ubiquitously expressed and their interaction is related to various diseases and health states such as migraines³ and Alzheimer's disease¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-CRLR/CALCRL (extracellular) Antibody (#ACR-060) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize CALCRL from human, mouse and rat samples.

Anti-CRLR/CALCRL (extracellular)-PE Antibody (#ACR-060-PE) is directly conjugated to R-Phycoerythrin (R-PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 22/07/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-PE (#RIC-001-PE), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to Phycoerythrin (PE) fluorophore.

Rabbit IgG Isotype Control-PE (#RIC-001-PE)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-CRLR/CALCRL (extracellular)-PE Antibody