Name: Anti-CRMP2 Antibody
Brand: Alomone Labs
SKU: AIP-029

Overview

Cat #: AIP-029

Alternative Name CRMP-2, Collapsin response mediator protein 2, Dihydropyrimidinase-related protein 2, DHPRP2, DRP-2, Dpysl2, Turned on after division 64 kDa protein, TOAD-64, Ulip2, Unc-33-like phosphoprotein 2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CEVSVTPKTVTPAS, corresponding to amino acid residues 504-517 of rat CRMP2 (Accession P47942). Intracellular.

Accession (Uniprot) Number P47942

Gene ID 25416

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human CCF-STTGI brain astrocytoma (lanes 3 and 7) and rat PC-12 peochromocytoma (lanes 4 and 8) cell line lysates:
1-3. Anti-CRMP2 Antibody (#AIP-029), (1:200).
4. Anti-CRMP2 Antibody (1:500).
5-8. Anti-CRMP2 Antibody, preincubated with CRMP2 Blocking Peptide (#BLP-IP029).

Immunohistochemistry

Expression of CRMP2 in rat spinal cord.
Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections sections with Anti-CRMP2 Antibody (#AIP-029), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CRMP2 immunoreactivity (green) appears in the superficial layers of the dorsal horn (arrows). B. Pre-incubation of the antibody with CRMP2 Blocking Peptide (BLP-IP029), suppressed staining. Cell nuclei are stained with DAPI (blue)

References

He, J. and Zhu, J. (2018) Med. Sci. Monit. 24, 4982.
Mokhtar, S.H. et al. (2018) Neural Regen. Res. 13, 1066.
Tan, M. et al. (2013) J. Neurochem. 125, 685.

Scientific background

Collapsin response mediator protein 2 (CRMP-2, DRP‐2), is a kinesin-binding protein and a member of the CRMP family, a novel family of microtubule-associated proteins (MAPs). This family consists of 5 highly conserved phosphorylated proteins: CRMP1-CRMP5. CRMP-2 was originally identified in the regulation of microtubule dimerization. CRMP-2 is the first member of the CRMP family identified in primary neuronal cells^1,2.

Physiological functions of CRMP-2 protein include regulation of cell surface receptor endocytosis, kinesin-dependent axonal transport, growth cone collapse, polarity and differentiation of developing neurons and neurite outgrowth and the regulation of microtubule dynamics².

Studies suggest that CRMP-2 can modulate neuronal viability through the PI3K/mTOR/S6K pathway. In addition, CRMP-2 expression has been detected in non-neuronal cells such as leukocytes, fibroblasts and neuroblastoma¹.

Phosphorylation of CRMP-2 on specific Thr and Ser residues inactivates its activity, leading to inhibition of axonal growth and neuronal polarity. Knockdown of CRMP‐2 shortens the total dendritic length and decreases dendritic branching³_.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-CRMP2 Antibody (#AIP-029) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize CRMP-2 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

CRMP2 Blocking Peptide (#BLP-IP029) is the original antigen used for immunization during Anti-CRMP2 Antibody (#AIP-029) generation. The blocking peptide binds and ‘blocks’ Anti-CRMP2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

CRMP2 Blocking Peptide (#BLP-IP029)

Shipping and Ordering Information

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Anti-CRMP2 Antibody