Overview
- Peptide (C)ELDLENFEYDDSAE, corresponding to amino acid residues 7- 20 of mouse CX3CR1 (Accession Q9Z0D9). Extracellular, N-terminus.
Cell surface detection of CX3CR1 by direct flow cytometry in live intact mouse BV-2 microglia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-APC (#RIC-001-APC).
___ Cells + Anti-CX3CR1 (extracellular)-APC Antibody (#ACR-058-APC), (2.5µg).
Cell surface detection of CX3CR1 by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-APC (#RIC-001-APC).
___ Cells + Anti-CX3CR1 (extracellular)-APC Antibody (#ACR-058-APC), (2.5µg).
- Bronger, H. et al. (2019) Cancer Metastasis Rev. 38, 417.
- Hamon, P. et al. (2017) Blood 129, 1296.
- Luo, P. et al. (2019) Brain. Res. Bull. 146, 12.
- Lee, M. et al. (2018) Immune Netw. 18, e5.
- Asai, H. et al. (2015) Nat. Neurosci. 18, 1584.
CX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.
CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3. Expression and interaction of CX3CR1 with CX3CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).
Both CX3CL1 and CX3CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX3CL1/CX3CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX3CL1/CX3CR1 signaling is associated with Alzheimer's Disease5. In the liver, CX3CR1 is expressed in monocytes, CD8+ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites4.
Anti-CX3CR1 (extracellular) Antibody (#ACR-058) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize CX3CR1 from mouse and rat samples. The antibody will not recognize human CX3CR1.
Anti-CX3CR1 (extracellular)-APC Antibody (#ACR-058-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize CX3CR1 from mouse and rat samples. The antibody will not recognize human CX3CR1.
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