Name: Anti-CX3CR1 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: ACR-058-F

Overview

Cat #: ACR-058-F

Alternative Name Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)ELDLENFEYDDSAE, corresponding to amino acid residues 7- 20 of mouse CX3CR1 (Accession Q9Z0D9). Extracellular, N-terminus.

Accession (Uniprot) Number Q9Z0D9

Gene ID 13051

Homology Rat – identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Specificity Not recommended for use with human samples.

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACR-058), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of CX3CR1 by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-CX3CR1 (extracellular)-FITC Antibody (#ACR-058-F), (2.5µg).
Cell surface detection of CX3CR1 by direct flow cytometry in live intact mouse BV-2 microglia cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-CX3CR1 (extracellular)-FITC Antibody (#ACR-058-F), (2.5µg).

References

Bronger, H. et al. (2019) Cancer Metastasis Rev. 38, 417.
Hamon, P. et al. (2017) Blood 129, 1296.
Luo, P. et al. (2019) Brain. Res. Bull. 146, 12.
Lee, M. et al. (2018) Immune Netw. 18, e5.
Asai, H. et al. (2015) Nat. Neurosci. 18, 1584.

Scientific background

CX₃C-chemokine receptor 1 (CX₃CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors¹.

CX₃CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX₃C chemokine ligand 1, (CX₃CL1). The major role of CX₃CR1 in immune cells is to recognize and enter inflamed tissue according to CX₃CL1 gradient and initiate the innate immune system². The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)³. Expression and interaction of CX₃CR1 with CX₃CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).

Both CX₃CL1 and CX₃CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX₃CL1/CX₃CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX₃CL1/CX₃CR1 signaling is associated with Alzheimer's Disease⁵. In the liver, CX₃CR1 is expressed in monocytes, CD8⁺ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-CX3CR1 (extracellular) Antibody (#ACR-058) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize CX3CR1 from mouse and rat samples. The antibody will not recognize human CX3CR1.

Anti-CX3CR1 (extracellular)-FITC Antibody (#ACR-058-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize CX3CR1 from mouse and rat samples. The antibody will not recognize human CX3CR1.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

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Anti-CX3CR1 (extracellular)-FITC Antibody