Name: Guinea pig Anti-D1 Dopamine Receptor Antibody
Brand: Alomone Labs
SKU: ADR-001-GP (formerly AGP-100)

Overview

Cat #: ADR-001-GP (formerly AGP-100)

Alternative Name DRD1, D(1A) dopamine receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: h, m, r

Immunogen

Peptide SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 (Accession P18901). Intracellular, C-terminus.

Accession (Uniprot) Number P18901

Gene ID 24316

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, dog, pig - identical; human - 13/14 amino acid residues identical.

RRID AB_2756622.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

$Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):$
Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):
1,2. Guinea pig Anti-D₁ Dopamine Receptor Antibody (#ADR-001-GP), (1:400).
3,4. Guinea pig Anti-D₁ Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
1. Guinea pig Anti-D₁ Dopamine Receptor Antibody (#ADR-001-GP), (1:400).
2. Guinea pig Anti-D₁ Dopamine Receptor Antibody, preincubated with D1 Dopamine Receptor Blocking Peptide (#BLP-DR001).

Immunohistochemistry

Expression of DRD1 in rat cortex
Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-D₁ Dopamine Receptor Antibody (#ADR-001-GP), (1:300), followed by goat-anti-guinea pig-Cy3 antibody. DRD1 staining (red) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue).

References

Missale, C. et al. (1998) Physiol. Rev. 78, 189.
Zeng, C. et al. (2008) Am. J. Physiol. 294, H551.

Scientific background

The D₁ dopamine receptor (D₁ receptor, DRD1) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and Schizophrenia¹.

All five dopamine receptors belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily.

Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D₁-like subfamily (that includes the D₁ and D₅ subtypes) and the D₂-like subfamily (that includes the D₂-, D₃- and D₄ subtypes)¹.

The D₁-like receptors are coupled to G_s-type G proteins and enhance adenylate cyclase activity while the D₂-like receptors are coupled to G_i-type G proteins and inhibit adenylate cyclase activity¹.

The D₁ receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex and striatum. In the periphery the D₁ receptor has been detected in the adrenal cortex, kidney and heart.

Functionally, the D₁ receptor has been implicated in the regulation of both locomotor and cognitive functions including the maintaining of spontaneous motor behaviors, the control of working memory and cognition as well as the regulation of craving and reward pathways.

In addition, the D₁ receptor plays an important role in the pathogenesis of hypertension by regulating epithelial Na⁺ transport and by interacting with vasoactive hormones/humoral factors, such as aldosterone and angiotensin^1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat D₁ Receptor. Guinea pig Anti-D₁ Dopamine Receptor Antibody (#ADR-001-GP) raised in guinea pigs can be used in western blot and immunohistochemistry applications. It has been designed to recognize DRD1 from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-D₁Dopamine Receptor Antibody (#ADR-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Immunohistochemistry citations

Rat spinal cord sections (1:75).
Rodgers, H.M. et al. (2019) Neuroscience 406, 376.

Immunofluorescence citations

Rat spinal cord sections (1:75).
Rodgers, H.M. et al. (2019) Neuroscience 406, 376.

Specifications

Scientific Background

Specific Control Product

D1 Dopamine Receptor Blocking Peptide (#BLP-DR001) is the original antigen used for immunization during Anti-D₁ Dopamine Receptor Antibody (#ADR-001) generation. The blocking peptide binds and ‘blocks’ Anti-D1 Dopamine Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

D1 Dopamine Receptor Blocking Peptide (#BLP-DR001)

Shipping and Ordering Information

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Guinea pig Anti-D₁ Dopamine Receptor Antibody