Name: Anti-D2 Dopamine Receptor (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: ADR-002-F

Overview

Cat #: ADR-002-F

Alternative Name DRD2, D(2) dopamine receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DDLERQNWSRPFNGSE, corresponding to amino acid residues 11-26 of rat DRD2 (Accession P61169). Extracellular, N-terminus.

Accession (Uniprot) Number P61169

Gene ID 24318

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 15/16 amino acid residues identical.

RRID AB_2756583.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label Fluorescein isothiocyanate (FITC).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ADR-002), and direct flow cytometry (labeled antibody).

Applications: fc, lci

May also work in: ic*

Direct flow cytometry

Cell surface detection of D₂ dopamine receptor in live intact human Jurkat T-cell leukemia cell line:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-D₂ Dopamine Receptor (extracellular)-FITC Antibody (#ADR-002-F), (5 µg antibody/0.5x10⁶ cells).
Cell surface detection of D₂ dopamine receptor in live intact mouse M1 myeloid leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-D₂ Dopamine Receptor (extracellular)-FITC Antibody (#ADR-002-F), (10 µg antibody/0.5x10⁶ cells).

References

Missale, C. et al. (1998) Physiol. Rev. 78, 189.
Kehne, J.H. et al. (2008) Curr. Top. Med. Chem. 8, 1068.

Scientific background

The D₂ dopamine receptor (DRD2) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and schizophrenia.¹

All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D₁-like subfamily (that includes the D₁ and D₅ subtypes) and the D₂-like subfamily (that includes the D₂-, D₃- and D₄ subtypes).¹

The D₁-like receptors are coupled to G_s-type G proteins and enhance adenylate cyclase activity while the D₂-like receptors are coupled to G_i-type G proteins and inhibit adenylate cyclase activity.¹

D₂ receptor expression in the brain is largely confined to the striatum, prefrontal cortex and hypothalamus.¹

Dysregulation of D₂ receptor function has been implicated in several disorders including schizophrenia, bipolar disorder, Parkinson's disease, and restless legs syndrome. Consistent with this, treatments that either block or activate the D₂ receptor have been developed to treat these diseases.²

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-D₂ Dopamine Receptor (extracellular) Antibody (#ADR-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize DRD2 from rat, human, and mouse samples.

Anti-D₂ Dopamine Receptor (extracellular)-FITC Antibody (#ADR-002-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-D₂ Dopamine Receptor (extracellular)-FITC Antibody