Name: Anti-D2 Dopamine Receptor (extracellular) Antibody
Brand: Alomone Labs
SKU: ADR-002

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Overview

Cat #: ADR-002

Alternative Name DRD2, D(2) dopamine receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DDLERQNWSRPFNGSE, corresponding to amino acid residues 11-26 of rat DRD2 (Accession P61169). Extracellular, N-terminus.

Accession (Uniprot) Number P61169

Gene ID 24318

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 15/16 amino acid residues identical.

RRID AB_2039828.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Rat striatum and hippocampus membranes (1:200) and rat and mouse whole brain membranes (1:200).
Western blot analysis of rat striatum (lanes 1 and 5) and hippocampus (lanes 2 and 6) membranes and of rat (lanes 3 and 7) and mouse (lanes 4 and 8) whole brain lysates:
1-4. Anti-D₂ Dopamine Receptor (extracellular) Antibody (#ADR-002), (1:200).
5-8. Anti-D₂ Dopamine Receptor (extracellular) Antibody, preincubated with D2 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR002).

Immunohistochemistry

Rat brain sections (1:100), (see Yang, Y.L. et al. (2019) Front. Neurosci. 13, 195.).

Scientific background

The D₂ dopamine receptor (DRD2) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and schizophrenia.¹

All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D₁-like subfamily (that includes the D₁ and D₅ subtypes) and the D₂-like subfamily (that includes the D₂-, D₃- and D₄ subtypes).¹

The D₁-like receptors are coupled to G_s-type G proteins and enhance adenylate cyclase activity while the D₂-like receptors are coupled to G_i-type G proteins and inhibit adenylate cyclase activity.¹

D₂ receptor expression in the brain is largely confined to the striatum, prefrontal cortex and hypothalamus.¹

Dysregulation of D₂ receptor function has been implicated in several disorders including schizophrenia, bipolar disorder, Parkinson's disease, and restless legs syndrome. Consistent with this, treatments that either block or activate the D₂ receptor have been developed to treat these diseases.²

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

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Certificate of Analysis

Anti-D₂ Dopamine Receptor (extracellular) Antibody