Name: Anti-DLGAP1/GKAP Antibody
Brand: Alomone Labs
SKU: APZ-041

Overview

Cat #: APZ-041

Alternative Name DLG associated protein 1, Discs large homolog associated protein 1, Guanylate kinase-associated protein, SAP90/PSD-95-associated protein 1, SAPAP1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KISNEHSPKLQIRSH, corresponding to amino acid residues 397-411 of rat DLGAP1 (Accession P97836). Intracellular.

Accession (Uniprot) Number P97836

Gene ID 65040

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – identical.

RRID AB_2756784.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ip*

Western blot

Mouse and rat brain lysates (1:200-1:1000).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
1,2. Anti-DLGAP1/GKAP Antibody (#APZ-041), (1:200).
3,4. Anti-DLGAP1/GKAP Antibody, preincubated with DLGAP1/GKAP Blocking Peptide (#BLP-PZ041).

Immunohistochemistry

Rat brain sections (1:200).

Scientific background

DLGAP1/GKAP (discs large homolog associated protein 1/guanylate kinase associated protein) is a member of the DLGAP family, scaffold proteins localized to the postsynaptic density. The protein is involved in signaling to and from glutamate receptors, it serves as a key adaptor protein of NMDA receptors, involved in learning and memory and synaptic plasticity in the central nervous system^1,2.

DLGAP family comprises five conserved proteins, DLGAP1–DLGAP5. DLGAP1-DLGAP4 proteins interact directly with both DLG and SHANK through their multiple domains. DLGAP1–4 proteins are implicated in several neurological disorders including schizophrenia, autism, obsessive compulsive disorder and cerebellar ataxia¹.

DLGAP1 is highly expressed in the brain; Dlgap1 mRNA is detected in the cortex, hippocampus, olfactory bulb, striatum and thalamus¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

DLGAP1/GKAP Blocking Peptide (#BLP-PZ041) is the original antigen used for immunization during Anti-DLGAP1/GKAP Antibody (#APZ-041) generation. The blocking peptide binds and ‘blocks’ Anti-DLGAP1/GKAP primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

DLGAP1/GKAP Blocking Peptide (#BLP-PZ041)

Shipping and Ordering Information

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Certificate of Analysis

Anti-DLGAP1/GKAP Antibody