Overview
- Peptide (C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT (Accession P23977). 2nd extracellular loop.
Cell surface detection of DAT in mouse J774 macrophages:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F), (5 µg).
Cell surface detection of DAT in mouse TK-1 T-cell lymphoma cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F), (5 µg).
- Robertson, S.D. et al. (2009) Mol. Neurobiol. 39, 73.
- Gainetdinov, R.R. (2008) Nauyn-Schiedeberg’s Arch. Pharmacol. 377, 301.
- Torres, G.E. and Amara, S.G. (2007) Curr. Opin. Neurobiol. 17, 304.
- Ciliax, B.J. et al. (1995) J. Neurosci. 15, 1714.
- Uhl, G.R. (2003) Mov. Disord. 18, S71.
The Na+/Cl- transporter family SLC6, includes DAT, a monoamine transporter, important for regulating extracellular levels of dopamine. It does so by taking up dopamine from the synaptic cleft via the co-transport of Na+ and Cl- down their electrochemical gradients1. The removal of dopamine by DAT remains the most important means to control the extracellular lifetime of the neurotransmitter and notably, the ending of dopaminergic neurotransmission2. Transporters for serotonin, norepinephrine, GABA and glycine also belong to this family3.
These receptors have 12 transmembrane spanning domains and intracellular N- and C-termini. DAT is also subject to post translational modifications such as phosphorylation, important for its regulation1. It also possesses a large extracellular domain which undergoes N-glycosylation, important for the proper targeting of the transporter to the plasma membrane1. DAT is expressed in dopaminergic cell bodies and terminals and can therefore serve as a marker for these neurons4. DAT is also expressed in the retina, gastrointestinal tract, lung, kidney, pancreas and lymphocytes2.
DAT plays an important role in movement as well as reward, learning and memory5. Its malfunction, which leads to dopaminergic dysregulation, has been associated with ADHD, schizophrenia, as well as Parkinson’s disease2. DAT is the main target for cocaine, amphetamine and methamphetamine psychostimulants, which mainly increase locomotor activities2.
Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize DAT from mouse and rat samples. The antibody is unlikely to recognize DAT from human samples.
Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.
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