Name: Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AER-051-F

Overview

Cat #: AER-051-F

Alternative Name EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.

Accession (Uniprot) Number Q14246

Gene ID 2015

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, Rat –11/14 amino acid residues identical.

RRID AB_2756589.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label Fluorescein isothiocyanate (FITC).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AER-051), and direct flow cytometry (labeled antibody).

Applications: fc

May also work in: ic*, lci*

Direct flow cytometry

Cell surface detection of EMR1 in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody (#AER-051-F), (2.5 µg).
Cell surface detection of EMR1 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody (#AER-051-F), (2.5 µg).

References

Legrand, F. et al. (2014) J. Allergy Clin. Immunol. 133, 1439.
McKnight, A.J. and Gordon, S. (1998) J. Leukoc. Biol. 63, 271.
Araç, D. et al. (2012) EMBO J. 31, 1364.

Scientific background

Human epidermal growth factor (EGF)–like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.

Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties^1,2.

EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations¹.

Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders^1,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell imaging applications. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize EMR1 from human, mouse, and rat samples.

Anti-EMR1 (ADGRE1) (extracellular)–FITC Antibody (#AER-051-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody