Name: Guinea pig Anti-Endothelin Receptor B Antibody
Brand: Alomone Labs
SKU: AER-002-GP

Overview

Cat #: AER-002-GP

Alternative Name EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, Endothelin receptor non-selective type

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: h, m, r

Immunogen

Peptide CEMLRKKSGMQIALND, corresponding to amino acid residues 298-314 of rat ET-B (Accession P21451). 3^rd intracellular loop.

Accession (Uniprot) Number P21451

Gene ID 50672

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - identical.

RRID AB_2756607.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.85 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
1,2. Guinea pig Anti-Endothelin Receptor B Antibody (#AER-002-GP), (1:500).
3,4. Guinea pig Anti-Endothelin Receptor B Antibody, preincubated with Endothelin Receptor B Blocking Peptide (#BLP-ER002).

References

Bender, S.B. et al. (2014) J. Physiol. 592, 1757.
Bramall, A.N. et al. (2013) PLoS ONE 8, e58023.
Jacques, D. et al. (2005) Peptides 26, 1427.

Scientific background

Endothelins (ET-1, 2, and 3) are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-Protein coupled receptors, ET_AR and ET_BR. Both ET_AR and ET_BR are present in the heart and in human myocardium at similar levels.^1,2

The endothelin receptors differ in their ligand specificity. While ET_AR has varying affinities for the endothelin isoforms (ET-1 > ET-2 > ET-3), ET_BR shows no selective affinity.^2,3

Subsequent studies have demonstrated the presence of endothelins in vascular, as well as in non-vascular, cells and tissues, having multiple biological activities.

Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.³

Until recently, it was thought that all cellular activities of the endothelins were mediated through their interactions with their cell surface receptors. However, a recent study demonstrated that cardiac nuclei also possess both ET_AR and ET_BR subtypes, which are functional and coupled to signaling mechanisms within the nuclear membrane.²

Hypermethylation of the ET_BR correlated with transcriptional down-regulation and reduced expression of ET_B receptor was observed in several prostate, bladder and colon cancer cell lines.³

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Guinea pig Anti-Endothelin Receptor B Antibody (#AER-002-GP, formerly AGP-037) is a highly specific antibody directed against an epitope of rat ET-B. The antibody can be used in western blot analysis. It has been designed to recognize ET-B from rat, mouse, and human samples. The antigen used to immunize guinea pigs is the same as Anti-Endothelin Receptor B Antibody (#AER-002) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 20/11/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Endothelin Receptor B Blocking Peptide (#BLP-ER002) is the original antigen used for immunization during Anti-Endothelin Receptor B Antibody (#AER-002) generation. The blocking peptide binds and ‘blocks’ Anti-Endothelin Receptor B primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Endothelin Receptor B Blocking Peptide (#BLP-ER002)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

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Guinea pig Anti-Endothelin Receptor B Antibody