Name: Anti-Ephrin-A5 (extracellular) Antibody
Brand: Alomone Labs
SKU: AER-035

Overview

Cat #: AER-035

Alternative Name EPH-related receptor tyrosine kinase ligand 7, LERK7, AL-1, EFNA5

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DRVFDVNDKVENSLE, corresponding to amino acid residues 172-186 of human Ephrin-A5 (Accession P52803). Extracellular.

Accession (Uniprot) Number P52803

Gene ID 1946

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat – identical.

RRID AB_2756586.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*, lci*

Western blot

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:
1,2. Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200).
3,4. Anti-Ephrin-A5 (extracellular) Antibody, preincubated with Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035).
Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:
1. Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200).
2. Anti-Ephrin-A5 (extracellular) Antibody, preincubated with Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035).

Immunohistochemistry

Expression of Ephrin-A5 rat hippocampus
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Ephrin A5 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (arrows) and in the granule layer (GL). Cell nuclei are stained with DAPI (blue).

References

Liu, Y. et al. (2018) PLoS One 13, e0198291.
Cheng, C. et al. (2017) Exp. Eye Res. 162, 9.
Das, G. et al. (2016) Cell Biosci. 6, 48.

Scientific background

The Eph family of tyrosine kinase receptor signaling pathway is important for a variety of physiological processes during development including cell morphology, proliferation, migration and axonal guidance, dendritic spine formation and neural plasticity. Eph receptors mediate forward signaling in one cell while ephrin ligands transmit reverse signaling in an adjacent cell^1-3_.

Eph receptors consist of two large subfamilies: EphA and EphB that bind to either glycophosphatidylinositol (GPI)-anchored ephrin-A ligands or transmembrane ephrin-B ligands respectively. Ephrin ligands are divided into ephrin-A (1 to 5) and ephrin-B (1 to 3)^1,2.

Ephrin-A5 is predominantly expressed in the quiescent anterior lens epithelial cells and is highly expressed in different layers of the cortex^2,3_.

Studies show that mutations in ephrin-A5 are linked to congenital and age-related cataracts in humans and mice_. Loss of ephrin-A5 causes abnormal localization of E-cadherin and β-catenin in anterior epithelial cells and epithelial-mesenchymal-transition².

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Ephrin-A5 (extracellular) Antibody (#AER-035) is a highly specific antibody directed against an epitope of human EFNA5. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize EFNA5 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035) is the original antigen used for immunization during Anti-Ephrin-A5 (extracellular) Antibody (#AER-035) generation. The blocking peptide binds and ‘blocks’ Anti-Ephrin-A5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Ephrin-A5 (extracellular) Blocking Peptide (#BLP-ER035)

Shipping and Ordering Information

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Anti-Ephrin-A5 (extracellular) Antibody