Name: Anti-SLC1A7 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGC-025

Overview

Cat #: AGC-025

Alternative Name Excitatory amino acid transporter 5, EAAT5, Retinal glutamate transporter

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)KETTEQSGKPVMS, corresponding to amino acid residues 122-134 of mouse EAAT5 (Accession Q8JZR4). 2^nd extracellular loop.

Accession (Uniprot) Number Q8JZR4

Gene ID 242607

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical; human – 12/13 amino acid residues identical.

RRID AB_2756598.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*, lci*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:
1,2. Anti-SLC1A7 (extracellular) Antibody (#AGC-025), (1:200).
3,4. Anti-SLC1A7 (extracellular) Antibody, preincubated with SLC1A7 (extracellular) Blocking Peptide (#BLP-GC025).

References

Scopelliti, A.J. et al. (2013) J. Biol. Chem. 288, 8250.
Benarroch, E.E. (2010) Neurology 74, 259.
Nelson, P.T. et al. (2013) Acta Neuropathol. 126, 161.

Scientific background

Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells throughout the body with glutamate for metabolic purposes.

SLC1A7 (EAAT5) belongs to The SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters¹. EAAT5 is expressed mainly in photoreceptors and bipolar cells in the retina and is essential for the maintenance of normal excitatory synaptic transmission as it determines the time course of glutamate receptor activation. EAAT5 consists of 3 identical subunits that are functionally coupled to the sodium (Na⁺), potassium (K⁺)-ATPase as part of the same macromolecular complex. The transporter operates via an “alternate access mechanism” whereby the translocation of one molecule of glutamate into the cell is coupled with the inward cotransport with 3Na⁺ and 1H⁺ ions. In turn, the return of the empty carrier to the extracellular side of the plasma membrane is coupled to the export of 1K⁺ ion. In addition, EAAT5, may function as glutamate-gated chloride (Cl⁻) channel, generating an anion current that is not stoichiometrically coupled to the uptake of glutamate².

EAAT5 has been linked to the development of hippocampal sclerosis. Hippocampal sclerosis of aging (HS-Aging) is a causative factor in a large proportion of elderly dementia cases³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-SLC1A7 (extracellular) Antibody (#AGC-025) is a highly selective antibody directed against an epitope of mouse Excitatory amino acid transporter 5. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize EAAT5 from mouse, human, and rat samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

SLC1A7 (extracellular) Blocking Peptide (#BLP-GC025) is the original antigen used for immunization during Anti-SLC1A7 (extracellular) Antibody (#AGC-025) generation. The blocking peptide binds and ‘blocks’ Anti-SLC1A7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

SLC1A7 (extracellular) Blocking Peptide (#BLP-GC025)

Shipping and Ordering Information

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Anti-SLC1A7 (extracellular) Antibody