Name: Anti-GABA Transporter 2 (GAT-2) Antibody
Brand: Alomone Labs
SKU: AGT-002

Overview

Cat #: AGT-002

Alternative Name SLC6A13, GABT2, Na⁺/Cl^-- dependent GABA transporter 2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)KVEEDGTLEREQWTNK, corresponding to amino acids residues 23-38 of rat GAT-2 (Accession P31646). Intracellular, N-terminus.

Accession (Uniprot) Number P31646

Gene ID 171163

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 14/16 amino acid residues identical; human - 11/16 amino acid residues identical.

RRID AB_10915893.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl,50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
1,2. Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002), (1:200).
3,4. Anti-GABA Transporter 2 (GAT-2) Antibody, preincubated with GABA Transporter 2/GAT-2 Blocking Peptide (#BLP-GT002).

Immunohistochemistry

Expression of GABA transporter 2 in rat lung
Immunohistochemical staining of rat lung paraffin-embedded sections using Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002), (1:100). GAT-2 (brown) is expressed in the respiratory epithelium of the bronchioli. Hematoxilin is used as the counterstain.

References

Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175.
Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375.
Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226.
Christiansen, B. et al. (2007) J. Biol. Chem. 282, 19331.

Scientific background

γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na⁺-dependent manner^1,2.

To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini¹. GATs couple the transport of one GABA molecule to 2 Na⁺ ions and one Cl^- ion³.

GAT-2 is expressed in the brain and in the periphery and is also abundant in the kidney and liver⁴. Due to its relatively broad expression pattern, suggested roles for GAT-2 include maturation of the nervous system, and regulation of GABA levels among others⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat GABA transporter 2. Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002) can be used in western blot analysis and immunohistochemistry applications and has been designed to recognize GAT-2 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

GABA Transporter 2/GAT-2 Blocking Peptide (#BLP-GT002) is the original antigen used for immunization during Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002) generation. The blocking peptide binds and ‘blocks’ Anti-GABA Transporter 2/GAT-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GABA Transporter 2/GAT-2 Blocking Peptide (#BLP-GT002)

Shipping and Ordering Information

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Anti-GABA Transporter 2 (GAT-2) Antibody