Name: Anti-GABA Transporter 3 (GAT-3) Antibody
Brand: Alomone Labs
SKU: AGT-003

Overview

Cat #: AGT-003

Alternative Name SLC6A11, GABT3, Na⁺/Cl^- -dependent GABA transporter 3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)REARDKAVHERGH, corresponding to amino acid residues 35-47 of rat GAT-3 (Accession P31647). Intracellular, N-terminus.

Accession (Uniprot) Number P31647

Gene ID 79213

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical.

RRID AB_2340977.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Not recommended for use with human samples.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:
1,2. Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003), (1:500).
3,4. Anti-GABA Transporter 3 (GAT-3) Antibody, preincubated with GABA Transporter 3/GAT-3 Blocking Peptide (#BLP-GT003).

References

Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175.
Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375.
Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226.
Kristensen, A.S. et al. (2011) Pharmacol. Rev. 63, 585.

Scientific background

γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na⁺-dependent manner^1,2.

To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini¹. GATs couple the transport of one GABA molecule to 2 Na⁺ ions and one Cl^- ion³.

GAT-3 is highly expressed in the brain and is linked to epilepsy⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GAT-3 from rat and mouse samples. The antibody will unlikely recognize the protein from human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Western blot citations

Rat cerebellum lysate.
Cabrera-Pastor, A. et al. (2019) Neuropharmacology 161, 107496.

Specifications

Scientific Background

Specific Control Product

GABA Transporter 3/GAT-3 Blocking Peptide (#BLP-GT003) is the original antigen used for immunization during Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003) generation. The blocking peptide binds and ‘blocks’ Anti-GABA Transporter 3/GAT-3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GABA Transporter 3/GAT-3 Blocking Peptide (#BLP-GT003)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-GABA Transporter 3 (GAT-3) Antibody