Overview
Alternative Name SLC6A11, GABT3, Na+/Cl- -dependent GABA transporter 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
- Peptide (C)REARDKAVHERGH, corresponding to amino acid residues 35-47 of rat GAT-3 (Accession P31647). Intracellular, N-terminus.
Accession (Uniprot) Number P31647
Gene ID 79213
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical.
RRID AB_2340977.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Specificity Not recommended for use with human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
- Rat and mouse brain lysate (1:500-1:1500).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:1,2. Anti-GABA Transporter 3 (GAT-3) Antibody (#AGT-003), (1:500).
3,4. Anti-GABA Transporter 3 (GAT-3) Antibody, preincubated with GABA Transporter 3/GAT-3 Blocking Peptide (#BLP-GT003).
Scientific background
γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.
To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3.
GAT-3 is highly expressed in the brain and is linked to epilepsy4.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
Citations
