Overview
- Peptide (C)GRYMGDRSIPHKK, corresponding to amino acid residues 413-425 of rat GABRB3 (Accession P63079). 2nd intracellular loop.
Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and LNCaP cell line lysate (lanes 3 and 6):1-3. Anti-GABA(A) β3 Receptor Antibody (#AGA-013), (1:1000)
4-6. Anti-GABA(A) β3 Receptor Antibody, preincubated with GABA(A) β3 Receptor Blocking Peptide (#BLP-GA013).
Expression of GABA(A) β3 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GABA(A) β3 Receptor Antibody (#AGA-013), (1:200), followed by goat anti-rabbit-Alexa-488. A. GABA(A) β3 immunoreactivity (green) appears in the cerebellar granule layer (G). B. Pre-incubation of the antibody with GABA(A) β3 Receptor Blocking Peptide (#BLP-GA013), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Middendorp, S.J. et al. (2015) Neuropharmacology 95, 459.
- Pomara, N. et al. (2015) Prog. Neuropsychopharmacol. Biol. Psychiatry 56, 35.
- Hentschke, H. et al. (2009) J. Neurophysiol. 102, 3643.
- Zhang, Y. et al. (2017) Medicine (Baltimore) 96, e9273.
- Khom, S. et al. (2016) J. Pharmacol. Exp. Ther. 357, 580.
GABA(A) receptors belong to the cys-loop pentameric ligand-gated ion channel family. These receptors are inhibitory neurotransmitter receptors in the brain and in the mammalian central nervous system. They are responsible for mediating GABA action, a neurotransmitter. They are composed of five homologous subunits that are arranged around a central chloride (Cl-)-selective pore. The human genome encodes 19 different GABA(A) receptor subunits: α1-6, β1-3, γ1-3, δ, ε, ρ1-3, π, and θ1,2,3.
The beta-3 subunit plays a crucial role in coordinating the network rhythms in the thalamus and olfactory bulb. The protein is responsible to controlling hyperexcitability, preventing seizures. GABA(A) β3 receptor expression is detected in dendritic regions of the hippocampus and dentate gyrus. The β3 subunit is also associated with slow GABAergic current kinetics3,4.
Mutation studies of GABRB3 gene are detected in patients with infantile spasms and Lennox–Gastaut syndrome. In addition, mice lacking the β3 subunit manifest fast decay kinetics on inhibitory postsynaptic currents in thalamic reticular and neocortical neurons3,5.
Anti-GABA(A) β3 Receptor Antibody (#AGA-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GABRB3 from human, rat, and mouse samples.
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