Name: Anti-GABA(A) γ1 Receptor Antibody
Brand: Alomone Labs
SKU: AGA-016

Overview

Cat #: AGA-016

Alternative Name GABRG1, Gamma-aminobutyric acid receptor subunit gamma-1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)HAGSTLIPMNNISMPQGE, corresponding to amino acid residues 382-399 of rat GABA(A) γ1 receptor (Accession P23574). Intracellular, C-terminus.

Accession (Uniprot) Number P23574

Gene ID 140674

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 16/18 amino acid residues identical; human - 14/18 amino acid residues identical.

RRID AB_2340941.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
1,2. Anti-GABA(A) γ1 Receptor Antibody (#AGA-016), (1:500).
3,4. Anti-GABA(A) γ1 Receptor Antibody, preincubated with GABA(A) γ1 Receptor Blocking Peptide (#BLP-GA016).

Immunohistochemistry

Expression of GABA(A) γ1 receptor in rat DRG
Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-GABA(A) γ1 Receptor Antibody (#AGA-016), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Staining (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.

References

Mihic, S.J. and Harris, R.A. (1994) Alcohol Health Res. World 21, 127.
Whiting, P.J. et al. (1999) Neurochem. Int. 34, 387.
Ashcroft, F.M. (2000) Ion Channels and Disease Ed 1.
Mehta, A.K. and Ticku, M.K. (1999) Brain Res. Rev. 29, 196.

Scientific background

The neurotransmitter GABA (γ-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABA_A receptor on these cells¹. There are two major types of GABA receptors: the ionotropic GABA_A (GABA_A R) and the metabotropic GABA_B receptors. GABA_A R belongs to the ligand gated ion channel superfamily^1,2.

The binding of GABA to its GABA_A receptor results in conformational changes that open a Cl^- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity^2,3.

They are composed of heteropentamer, with all of the five subunits contributing to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ¹. The native GABA_A receptor, in most cases, consists of 2α, 2β and 1γ subunit. Three γ subunits genes have been identified in mammals.

mRNA of the γ1 subunit is detected in the amygdala and septum⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GABA(A) γ1 Receptor Antibody (#AGA-016) is a highly specific antibody directed against an epitope of rat GABRG1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABA(A) γ1 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

KO validation citations

Immunocytochemistry staining of rat cultured oligodendrocyte progenitor cells. Tested in cells treated with GABA(A)γ1 siRNA.
Ordaz, R.P. et al. (2021) Mol. Pharmacol. 99, 133.

Specifications

Scientific Background

Specific Control Product

GABA(A) γ1 Receptor Blocking Peptide (#BLP-GA016) is the original antigen used for immunization during Anti-GABA(A) γ1 Receptor Antibody (#AGA-016) generation. The blocking peptide binds and ‘blocks’ Anti-GABA(A) γ1 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GABA(A) γ1 Receptor Blocking Peptide (#BLP-GA016)

Shipping and Ordering Information

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Anti-GABA(A) γ1 Receptor Antibody