Overview
- Peptide QKSDDDYEDYASNKT(C), corresponding to amino acid residues 39-53 of rat GABA(A) γ2 receptor (Accession P18508). Extracellular, N-terminus.
Western blot analysis of rat brain membranes:1. Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005), (1:200).
2. Anti-GABA(A) γ2 Receptor (extracellular) Antibody, preincubated with GABA(A) γ2 Receptor (extracellular) Blocking Peptide (#BLP-GA005).- Transfected cells with human GABRG2 (Kang, J.Q. et al. (2010) J. Neurosci. 30, 13895.).
- Rat brain formalin-fixed frozen section.
- Rat adrenal medullary cells (1:100) (Matsuoka, H. et al. (2008) J. Physiol. 586, 4825.).
- Mouse spinal cord neurons (1:100) (Shrivastava, A.N. et al. (2011) J. Biol. Chem. 286, 14455.).
- Mouse hippocampal neurons (1:100) (Chou, W.H. et al. (2010) J. Neurosci. 30, 13955.).
- Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127.
- Whiting, P.J. et al. (1999) Neurochem. Int. 34, 379.
- Ashcroft, F.M. (2000) Ion Channels and Disease Ed 1.
- Wallace, R.H. et al. (2001) Nat. Genet. 28, 49.
- Baulac, S. et al. (2001) Nat. Genet. 28, 46.
The neurotransmitter GABA (γ-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 There are two major types of GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors.
GABAA R belongs to the ligand gated ion channel superfamily.1,2
It is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1
The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ subunit. Three γ subunits genes have been identified in mammals.
The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity.2,3
Recently, a genetic linkage between familial epilepsy syndrome and mutations in the γ2 subunit of the GABAA receptor have been demonstrated.4,5
Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005) is a highly specific antibody directed against an epitope of rat GABRG2. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. The antibody recognizes an extracellular epitope and is thus suited for live cell imaging. It has been designed to recognize GABA(A) γ2 from human, rat, and mouse samples.
![Anti-GABA(A) γ2 Receptor (extracellular) Antibody](https://www.alomone.com/wp-content/uploads/2016/09/aga-005.jpg" "Anti-GABA(A) γ2 Receptor (extracellular) Antibody")
TNF-α-induced downregulation of surface GABA(A) γ2 Receptor.Immunocytochemical staining of rat living hippocampal neurons. A. Extracellular staining of cells using Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005), (1:100). Representative images from time course experiments with cultured neurons undergoing TNF-α treatment ranging from 10 min to 6 h in duration. Results show a rapid downregulation in surface levels of the γ2 subunit in response to TNF-α. B. Quantification data of surface γ2 cluster area, over the 6 h time course of TNF-α treatment (n = 120 –158 images per time point; one-way ANOVA, Tukey’s post hoc test, **p < 0.01, ***p < 0.001).Adapted from Pribiag, H. and Stellwagen, D. (2013) with permission of the Society for Neuroscience.Applications
Citations
Powered by Bioz- Mouse brain lysate (1:300).
Du, Z. et al. (2016) Neuroscience 329, 363. - HEK 293 transfected cells.
Wang, J. et al. (2016) Sci. Rep. 6, 35294. - HEK 293T transfected cells (1:100).
Warner, T.A. et al. (2016) Hum. Mol. Genet. 25, 3192. - Mouse brain lysate.
Lo, W.Y. et al. (2014) Neurochem. Res. 39, 1088. - Mouse brain lysate (1:200).
Vlachos, A. et al. (2013) Cereb. Cortex 23, 2700.
- Rat living primary hippocampal neurons (1:100).
Levi, S. et al. (2015) Neuropharmacology 88, 199. - Rat living hippocampal neurons (1:100).
Pribiag, H. and Stellwagen, D. (2013) J. Neurosci. 33, 15879. - Mouse spinal cord neurons (1:100).
Shrivastava, A.N. et al. (2011) J. Biol. Chem. 286, 14455. - Mouse hippocampal neurons (1:100).
Chou, W.H. et al. (2010) J. Neurosci. 30, 13955.
- Mouse spinal cord sections.
Cantaut-Belarif, Y. et al. (2017) J. Cell Biol. 216, 2979.
- Mouse primary spinal cord neurons.
Cantaut-Belarif, Y. et al. (2017) J. Cell. Biol. 216, 2979. - Rat granule cells.
Bazzurro, V. et al. (2018) J. Mol. Neurosci. 64, 312. - Rat adrenal medullary cells (1:100).
Matsuoka, H. et al. (2008) J. Physiol. 586, 4825.
- Yue, J. et al. (2018) Brain Res. Bull. 137, 156.
- de Luca, E. et al. (2017) Neuron 95, 63.
- Kang, J.Q. et al. (2010) J. Neurosci. 30, 13895.
