Overview
- Peptide QKSDDDYEDYASNKT(C), corresponding to amino acid residues 39-53 of rat GABA(A) γ2 receptor (Accession P18508). Extracellular, N-terminus.
- Rat brain membrane (1:200). Transfected cells with human GABRG2 (Kang, J.Q. et al. (2010) J. Neurosci. 30, 13895.).
Western blot analysis of rat brain membranes:1. Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005), (1:200).
2. Anti-GABA(A) γ2 Receptor (extracellular) Antibody, preincubated with GABA(A) γ2 Receptor (extracellular) Blocking Peptide (#BLP-GA005).
- Rat brain formalin-fixed frozen section.
- Rat adrenal medullary cells (1:100) (Matsuoka, H. et al. (2008) J. Physiol. 586, 4825.).
- Mouse spinal cord neurons (1:100) (Shrivastava, A.N. et al. (2011) J. Biol. Chem. 286, 14455.).
- Mouse hippocampal neurons (1:100) (Chou, W.H. et al. (2010) J. Neurosci. 30, 13955.).
The neurotransmitter GABA (γ-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 There are two major types of GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors.
GABAA R belongs to the ligand gated ion channel superfamily.1,2
It is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1
The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ subunit. Three γ subunits genes have been identified in mammals.
The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity.2,3
Recently, a genetic linkage between familial epilepsy syndrome and mutations in the γ2 subunit of the GABAA receptor have been demonstrated.4,5
![Anti-GABA(A) γ2 Receptor (extracellular) Antibody](https://www.alomone.com/wp-content/uploads/2016/09/aga-005.jpg" "Anti-GABA(A) γ2 Receptor (extracellular) Antibody")
TNF-α-induced downregulation of surface GABA(A) γ2 Receptor.Immunocytochemical staining of rat living hippocampal neurons. A. Extracellular staining of cells using Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005), (1:100). Representative images from time course experiments with cultured neurons undergoing TNF-α treatment ranging from 10 min to 6 h in duration. Results show a rapid downregulation in surface levels of the γ2 subunit in response to TNF-α. B. Quantification data of surface γ2 cluster area, over the 6 h time course of TNF-α treatment (n = 120 –158 images per time point; one-way ANOVA, Tukey’s post hoc test, **p < 0.01, ***p < 0.001).Adapted from Pribiag, H. and Stellwagen, D. (2013) with permission of the Society for Neuroscience.
