Overview
- Peptide CKDPIEDINSPEHIQRRLSL, corresponding to amino acid residues 875-894 of human GABBR2 (Accession O75899). Intracellular, C-terminus.
Western blot analysis of rat brain membrane:1. Anti-GABA(B) R2 Antibody (#AGB-002), (1:200).
2. Anti-GABA(B) R2 Antibody, preincubated with GABA(B) R2 Blocking Peptide (#BLP-GB002).
Expression of GABA(B) receptor 2 in mouse hippocampusImmunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R2 Antibody (#AGB-002 ), (1:100). A. GABBR2 staining (green) is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red) sets apart the SLM. C. Confocal merge suggests the presence of GABBR2 in layers, but not in pyramidal neurons.
- Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109.
- Bettler, B. et al. (2004) Physiol. Rev. 84, 835.
- Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035.
- Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043.
- Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14649.
- Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14643.
- http://www.uniprot.org/uniprot/O75899.
GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2
The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA(B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor (GPCR) superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4
The functional GABA(B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6
GABA(B) R2 has been shown to be essential for trafficking and for G-protein binding while the GABA(B) R1 subunit has been demonstrated to be important for agonist and antagonist binding. GABA(B) R2 is highly expressed in the brain, especially in the cerebral cortex, thalamus, and hippocampus, spinal cord, and, to a lesser extent, in the testis.7
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human GABA(B) receptor 2. Anti-GABA(B) R2 Antibody (#AGB-002), can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABBR2 from human, rat, and mouse samples.
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