Overview
- Peptide(C)GSGSQRAKRKVTR, corresponding to amino acid residues 223-235 of mouse Galanin receptor type 2 (Accession O88854). 3rd intracellular loop.
Western blot analysis of rat hippocampus (lanes 1 and 4), rat DRG (lanes 2 and 5), rat brain (lanes 3 and 6), mouse brain (lanes 7 and 9) and human prostate carcinoma LNCaP cell lysates (lanes 8 and 10):1,2,3,7,8. Anti-GALR2 Antibody (#AGR-012), (1:200).
4,5,6,9,10. Anti-GALR2 Antibody, preincubated with GALR2 Blocking Peptide (#BLP-GR012).
Expression of Galanin receptor 2 in rat DRGImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-GALR2 Antibody (#AGR-012), (1:100). GALR2 is expressed in the DRG neurons. Hoechst 33342 is used as the counterstain.
- Branchek, T.A. et al. (2000) Trends Pharmacol Sci. 21, 109.
- Branchek, T.A. et al. (1998) Ann. N. Y. Acad. Sci. 863, 94.
- Barreda-Gómez, G. et al. (2005) Neuropeptides 39, 153.
- Lu, X. et al. (2005) Neuropeptides 39, 147.
- Counts, S.E. et al. (2003) Mol. Interv. 3, 137.
Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4
GALR2 is widely expressed in the brain (with highest levels of mRNA found in the hypothalamus, hippocampus, amygdala and pyriform cortex) and in peripheral tissues such as the small intestine, heart, kidney, and liver. High levels of GALR2 expression were also found in rat dorsal root ganglia.1
GALR2 activation increases inositol phosphate hydrolysis, mediates the release of Ca2+ into the cytoplasm from intracellular stores, and opens Ca2+-dependent chloride channels in a PTX-resistant manner, indicating that GALR2 couples to Gq/11 proteins.5
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse Galanin receptor 2. Anti-GALR2 Antibody (#AGR-012) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GALR2 from mouse, rat and human samples.
Applications
Citations
Powered by Bioz- Rat brain sections.
Flores-Burgess, A. et al. (2019) Neuropharmacology 155, 104. - Rat brain sections.
Narvaez, M. et al. (2018) Front. Cell. Neurosci. 12, 119. - Human periprostatic neurovascular tissue sections.
Weyne, E. et al. (2018) J. Sex. Med. 15, 480. - Rat brain sections.
Millon, C. et al. (2016) Brain Struct. Funct. 221, 4491. - Rat brain sections (1:100).
Narvaez, M. et al. (2015) Brain Struct. Funct. 221, 4129. - Rat brain sections (1:250).
Millon, C. et al. (2014) Int. J. Neuropsychopharmacol. 18, pyu064.
- Rat brain sections.
Flores-Burgess, A. et al. (2019) Neuropharmacology 155, 104. - Rat brain sections.
Narvaez, M. et al. (2018) Front. Cell. Neurosci. 12, 119. - Human periprostatic neurovascular tissue sections.
Weyne, E. et al. (2018) J. Sex. Med. 15, 480. - Rat brain sections.
Millon, C. et al. (2016) Brain Struct. Funct. 221, 4491. - Rat brain sections (1:100).
Narvaez, M. et al. (2015) Brain Struct. Funct. 221, 4129.
