Overview
- Peptide(C)GSGSQRAKRKVTR, corresponding to amino acid residues 223-235 of mouse Galanin receptor type 2 (Accession O88854). 3rd intracellular loop.
- Rat brain, hippocampus and DRG lysates; Mouse brain and human prostate carcinoma cell line LNCaP lysates (1:200).
Western blot analysis of rat hippocampus (lanes 1 and 4), rat DRG (lanes 2 and 5), rat brain (lanes 3 and 6), mouse brain (lanes 7 and 9) and human prostate carcinoma LNCaP cell lysates (lanes 8 and 10):1,2,3,7,8. Anti-GALR2 Antibody (#AGR-012), (1:200).
4,5,6,9,10. Anti-GALR2 Antibody, preincubated with GALR2 Blocking Peptide (#BLP-GR012).
- Rat DRG frozen sections (1:100).
Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4
GALR2 is widely expressed in the brain (with highest levels of mRNA found in the hypothalamus, hippocampus, amygdala and pyriform cortex) and in peripheral tissues such as the small intestine, heart, kidney, and liver. High levels of GALR2 expression were also found in rat dorsal root ganglia.1
GALR2 activation increases inositol phosphate hydrolysis, mediates the release of Ca2+ into the cytoplasm from intracellular stores, and opens Ca2+-dependent chloride channels in a PTX-resistant manner, indicating that GALR2 couples to Gq/11 proteins.5
