Name: Anti-GALR3 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-013

Overview

Cat #: AGR-013

Alternative Name Galanin receptor type 3, GALNR3, GAL3R

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide SYYGTVRYGALELC, corresponding to amino acid residues 159-172 of mouse Galanin receptor type 3 (Accession O88853). 2^nd extracellular loop.

Accession (Uniprot) Number O88853

Gene ID 14429

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, human - identical.

RRID AB_2039874.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.7 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain membrane:
1. Anti-GALR3 (extracellular) Antibody (#AGR-013), (1:200).
2. Anti-GALR3 (extracellular) Antibody, preincubated with GALR3 (extracellular) Blocking Peptide (#BLP-GR013).

Immunohistochemistry

Expression of Galanin receptor 3 in rat hippocampus
Immunohistochemical staining of rat hippocampus using Anti-GALR3 (extracellular) Antibody (#AGR-013). A. Galanin receptor 3 (green) appears in apical dendrites of pyramidal neurons in CA1 (arrows). B. MAP2 (red), a marker of axons and dendrites, is stained in the same section. GALR3 appears to be expressed in a subset of apical dendrites (arrows). DAPI is used as the counterstain (blue).

Indirect flow cytometry

Cell surface detection of Galanin receptor 3 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-GALR3 (extracellular) Antibody (#AGR-013), (2.5μg) + goat-anti-rabbit-APC.

References

Branchek, T.A. et al. (2000) Trends Pharmacol Sci. 21, 109.
Branchek, T.A. et al. (1998) Ann. N. Y. Acad. Sci. 863, 94.
Barreda-Gómez, G. et al. (2005) Neuropeptides 39, 153.
Lu, X. et al. (2005) Neuropeptides 39, 147.
Hawes, J.J. et al. (2004) J. Comp. Neurol. 479, 410.

Scientific background

Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.^1-4

Based on mRNA, the GALR1 is predominantly distributed in the central nervous system, spinal cord and dorsal root ganglia while GALR2 is abundant in both central and peripheral nervous systems. GALR3 mRNA is distributed in peripheral tissues such as the heart, spleen, and testis. However, its expression in the brain is still controversial.^4,5

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GALR3 (extracellular) Antibody (#AGR-013) is a highly specific antibody directed against an epitope of the mouse Galanin receptor 3. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GALR3 from mouse, rat, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Immunohistochemistry citations

Human periprostatic neurovascular tissue sections.
Weyne, E. et al. (2018) J. Sex. Med. 15, 480.

Immunofluorescence citations

Human periprostatic neurovascular tissue sections.
Weyne, E. et al. (2018) J. Sex. Med. 15, 480.

Specifications

Scientific Background

Specific Control Product

GALR3 (extracellular) Blocking Peptide (#BLP-GR013) is the original antigen used for immunization during Anti-GALR3 (extracellular) Antibody (#AGR-013) generation. The blocking peptide binds and ‘blocks’ Anti-GALR3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GALR3 (extracellular) Blocking Peptide (#BLP-GR013)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Anti-GALR3 (extracellular) Antibody