Overview
Alternative Name Galanin receptor type 3, GALNR3, GAL3R
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide SYYGTVRYGALELC, corresponding to amino acid residues 159-172 of mouse Galanin receptor type 3 (Accession O88853). 2nd extracellular loop.
Accession (Uniprot) Number O88853
Gene ID 14429
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human - identical.
RRID AB_2039874.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.7 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
- Rat brain membrane (1:200).
Western blot analysis of rat brain membrane:1. Anti-GALR3 (extracellular) Antibody (#AGR-013), (1:200).
2. Anti-GALR3 (extracellular) Antibody, preincubated with GALR3 (extracellular) Blocking Peptide (#BLP-GR013).
Immunohistochemistry
- Rat hippocampus.
Indirect flow cytometry
Cell surface detection of Galanin receptor 3 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-APC.
___ Cells + Anti-GALR3 (extracellular) Antibody (#AGR-013), (2.5μg) + goat-anti-rabbit-APC.
Scientific background
Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4
Based on mRNA, the GALR1 is predominantly distributed in the central nervous system, spinal cord and dorsal root ganglia while GALR2 is abundant in both central and peripheral nervous systems. GALR3 mRNA is distributed in peripheral tissues such as the heart, spleen, and testis. However, its expression in the brain is still controversial.4,5
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
Citations
