Overview
- Peptide (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43 (Accession P07936). C-terminus.
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2 Anti-GAP43 Antibody (#ANR-016), (1:400).
3,4. Anti-GAP43 Antibody, preincubated with GAP43 Blocking Peptide (#BLP-NR016).
Expression of GAP43 in rat hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GAP43 Antibody (#ANR-016), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GAP43 staining (green) appears in a layer bordering granule layer (G), (arrow). B. Calbindin D28k staining (red) is detected in granule layer (G) neurons. C. Merge of the two images demonstrates distinct spatial localizations in the hippocampal dentate gyrus. Cell nuclei are stained with DAPI (blue).
- Li, Y. et al. (2017) Braz. J. Med. Biol. Res. 50, e5561.
- Grasselli, G. and Strata, P. (2013) Front. Neural Circuits. 7, 25.
Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.
GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.
Anti-GAP43 Antibody (#ANR-016) is a highly selective antibody directed against an epitope of rat Neuromodulin. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from human, mouse, and rat samples.
Powered by Bioz
