Name: Anti-GIPC1 Antibody
Brand: Alomone Labs
SKU: APZ-045

Overview

Cat #: APZ-045

Alternative Name PDZ domain-containing protein GIPC1, GIPC, GAIP C-terminus-interacting protein, RGS-GAIP-interacting protein, Synectin, NIP, TIP-2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KAPPLVENEEAEPGR, corresponding to amino acid residues 10-24 of human GIPC1 (Accession O14908). Intracellular, N-terminus.

Accession (Uniprot) Number O14908

Gene ID 10755

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse – 14/15 amino acid residues identical.

RRID AB_2756785.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
1,2. Anti-GIPC1 Antibody (#APZ-045), (1:400).
3,4. Anti-GIPC1 Antibody, preincubated with GIPC1 Blocking Peptide (#BLP-PZ045).
Western blot analysis of human U87-MG glioblastoma cell line lysate:
1. Anti-GIPC1 Antibody (#APZ-045), (1:400).
2. Anti-GIPC1 Antibody, preincubated with GIPC1 Blocking Peptide (#BLP-PZ045).

Immunohistochemistry

Expression of GIPC1 in mouse and rat hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections with Anti-GIPC1 Antibody (#APZ-045), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. In mouse brain GIPC1 staining (green) appears in neurons of the pyramidal layer (P) and their apical dendrites (arrows). B. In rat brain similar staining is observed. Cell nuclei are stained with DAPI (blue).

References

Shang, G. et al. (2017) eLife 6, e27322.
Katoh, M. (2013) Exp. Mol. Med. 45, e26.

Scientific background

GIPC1 belongs to a family of evolutionarily conserved adaptor proteins responsible for the trafficking of numerous transmembrane proteins via endocytosis. Members of GIPC (GAIP interacting protein, C-terminus) have three main domains responsible for protein interactions; a PDZ domain which is flanked by GH1 and GH2 domains. GH1 domain is responsible for the dimerization of the protein, while GH2 is important for the interaction of GIPC1 with MYO6, a retrograde motor protein involved in protein trafficking. The PDZ domain of GIPC1 is responsible for the protein to interact with numerous proteins; namely β₁-adrenoceptor, D₂ dopamine receptor, GLUT1, TrkA receptor just to name a few. The zebrafish ortholog also interacts with Neuropilin-2^1,2.

GIPC1 expression is upregulated in breast, ovarian and pancreas cancers².

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GIPC1 Antibody (#APZ-045) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GIPC1 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

GIPC1 Blocking Peptide (#BLP-PZ045) is the original antigen used for immunization during Anti-GIPC1 Antibody (#APZ-045) generation. The blocking peptide binds and ‘blocks’ Anti-GIPC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GIPC1 Blocking Peptide (#BLP-PZ045)

Shipping and Ordering Information

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Anti-GIPC1 Antibody