Anti-GLP1R (extracellular)-PE Antibody

Overview

Cat #: AGR-021-PE

Alternative Name Glucagon-like peptide 1 receptor, GLP-1 receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: H, M, R

Immunogen

Peptide (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R (Accession P32301). 2^nd extracellular loop.

Accession (Uniprot) Number P32301

Gene ID 25051

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse, human - 13/14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label R-Phycoerythrin (R-PE).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-021), and direct flow cytometry (labeled antibody).

Applications: FC, LCI

Direct flow cytometry

Cell surface detection of Glucagon-like peptide 1 receptor by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-PE (#RIC-001-PE).
^___ Cells + Anti-GLP1R (extracellular)-PE Antibody (#AGR-021-PE), (2.5µg).
Cell surface detection of Glucagon-like peptide 1 receptor by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-PE (#RIC-001-PE).
^___ Cells + Anti-GLP1R (extracellular)-PE Antibody (#AGR-021-PE), (5µg).

References

Donath, M.Y. et al. (2003) J. Mol. Med. 81, 455.
Buteau, J. (2008) Diabetes Metab. 34, S73.
Holst, J.J. (2007) Physiol. Rev. 87, 1409.
Harkavyi, A. and Whitton, P.S. (2010) Br. J. Pharmacol. 159, 495.

Scientific background

Glucagon-like peptide 1 (GLP-1) is a hormone secreted by the intestine following a meal¹. This hormone is known to enhance the secretion of insulin, important glucose blood level regulation^2,3. In pancreatic β-cells, other than stimulating insulin secretion, GLP-1 action is also important for β-cell mass expansion².

GLP-1 exerts its biological functions through Glucagon-like peptide 1 receptor (GLP-1 receptor), a member of the G-protein coupled receptor superfamily. Like all members, this receptor spans the membrane seven times. GLP-1 receptor couples with G_s, thereby stimulating adenylate cyclase, consequently leading to an increase in cAMP².

The receptor is expressed in various tissues, namely the pancreas, adipose tissue, muscle, heart, gastrointestinal tract and liver. GLP-1 receptor is also found in the central nervous system where it provides a neuroprotective effect⁴.

The glucagon-like peptide 1 system may serve as a therapeutic treatment in diabetes mellitus, in order to maintain β-cell mass expansion². In addition, the receptor may serve as a therapy target in various neurodegenerative diseases like Alzheimer’s and Parkinson’s disease⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GLP1R (extracellular) Antibody (#AGR-021) is a highly specific antibody directed against an epitope of the rat glucagon-like peptide 1 receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize GLP1R from human, rat, and mouse samples.

Anti-GLP1R (extracellular)-PE Antibody (#AGR-021-PE) is directly conjugated to R-Phycoerythrin (R-PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 19/02/2025

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-PE (#RIC-001-PE), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to Phycoerythrin (PE) fluorophore.

Rabbit IgG Isotype Control-PE (#RIC-001-PE)

Shipping and Ordering Information

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