Name: Anti-GPR119 Antibody
Brand: Alomone Labs
SKU: AGR-032

Overview

Cat #: AGR-032

Alternative Name Glucose-dependent insulinotropic receptor, GPCR2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide CDMLK IASVHSQHIR, corresponding to amino acid residues 186-200 of rat GPR119 (Accession Q7TQN8). 2^nd intracellular loop.

Accession (Uniprot) Number Q7TQN8

Gene ID 302813

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.

RRID AB_2756629.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.85 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat pancreas (lanes 1 and 3) and mouse MS1 cells (lanes 2 and 4):
1,2. Anti-GPR119 Antibody (#AGR-032), (1:400).
3,4. Anti-GPR119 Antibody, preincubated with GPR119 Blocking Peptide (#BLP-GR032).

References

Griffin, G. (2006) Methods for identification of modulators of OSGPR116 activity.
Fredriksson, R. et al. (2003) FEBS Lett. 554, 381.
Overton, H.A. et al. (2007) Br, J, Pharmacol, 153, S76.
Ohishi, T. and Yoshida, S. (2012) Expert Opin. Investig. Drugs 21, 321.
Hansen, H.S. et al. (2012) Trends Pharmacol. Sci. 33, 374.

Scientific background

Glucose-dependent insulinotropic receptor (GPR119) is a rhodopsin-type member of the G protein-coupled receptor (GPCRs) superfamily. Like all members, it has seven transmembrane spanning domains, an extracellular N-terminus and intracellular C-terminus. Activation of the receptor leads to a rise in intracellular cAMP – suggesting a stimulation of adenylate cyclase, possibly by G_αs coupling. It is expressed mostly in enteroendocrine cells of the gastrointenstinal tract and b-cells of the pancreas. It was stipulated that GPR119 is a close relative to the cannabinoid receptors^1,2.

Phospholipids and fatty acid amides are endogenous ligands for GPR119. Notably, oleoethanolamide (OEA) seems to be an important agonist. Stimulation of GPR119 by its agonists causes b-cells to release insulin and enteroendocrine cells to release glucagon-like peptide-1 (GLP-1); this attribute makes GPR119 a strong therapeutic candidate for type-2 diabetes and obesity^3,4. Furthermore, its ability to release GLP-1 upon stimulation by dietary fat has gained it the title "fat sensor"⁵ .

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Glucose-dependent insulinotropic receptor. Anti-GPR119 Antibody (#AGR-032) can be used in western blot analysis. It has been designed to recognize GPR119 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

GPR119 Blocking Peptide (#BLP-GR032) is the original antigen used for immunization during Anti-GPR119 Antibody (#AGR-032) generation. The blocking peptide binds and ‘blocks’ Anti-GPR119 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR119 Blocking Peptide (#BLP-GR032)

Shipping and Ordering Information

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Anti-GPR119 Antibody