Overview
- Peptide (C)KPSTELEYLGPDE, corresponding to amino acid residues 495-507 of human Glucose Transporter 4 (Accession P14672). Intracellular, C-terminus.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes:1,2. Anti-GLUT4 Antibody (#AGT-024), (1:400).
3,4. Anti-GLUT4 Antibody, preincubated with GLUT4 Blocking Peptide (#BLP-GT024).
Expression of Glucose Transporter 4 in rat olfactory bulbImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GLUT4 Antibody (#AGT-024), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. GLUT4 staining (green) appears in some glomeruli (“G” and horizontal arrows), but not in others (vertical arrow). Cell nuclei are stained with DAPI (blue).
- Lacombe, V.A. (2014) ISRN Vet Sci. 2014, 409547.
- Stöckli, J. et al. (2011) J. Cell Sci. 124, 41472.
The GLUT protein family is a member of the major facilitator superfamily of membrane transporters and is encoded by the SLC2 genes. GLUT proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini. In addition there are several conserved residues and motifs designated “sugar transporter signatures”.
To date, 14 GLUT isoforms have been identified and divided into 3 different classes based on sequence similarity and structural and functional characteristics. GLUT4 together with GLUT 1-3 and 14 belong to Class I.
GLUT4 is an insulin-regulated glucose transporter responsible for insulin-regulated glucose uptake into fat and muscle cells. GLUT4 translocates from intracellular stores to the cell surface, from an inactive site to an active one in order to enhance glucose uptake1.
GLUT4 is expressed mainly in adipose tissue and skeletal muscle. It is also detected in brain, kidney and intestine.
There is no evidence for mutation in GLUT4 that is linked to metabolic disease in humans. However, GLUT4 polymorphisms are rare and equally common in normoglycaemic individuals and type 2 diabetics. General decrease in GLUT4 levels show signs of diabetes, while a disruption in GLUT4 expression in muscle or adipose tissue might contribute to the development of whole-body insulin resistance in humans2.
Anti-GLUT4 Antibody (#AGT-024) is a highly specific antibody directed against an epitope of the human Glucose Transporter 4 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT4 from human, rat, and mouse samples.
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