Overview
- Peptide (C)KNKVSDVYPEKEEK, corresponding to amino acid residues 477 - 490 of rat Glucose Transporter 5 (Accession P43427). Intracellular, C-terminus.
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:400).
3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
Western blot analysis of rat small intestine lysate:1. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
2. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
Western blot analysis of human MCF-7 breast adenocarcinoma cell lysates (lanes 1 and 3) and human Caco-2 colon adenocarcinoma cell lysates (lanes 2 and 4):1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
Expression of Glucose Transporter 5 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GLUT5 staining (green) in hippocampal CA3 region, is detected in cells of the pyramidal layer (P) and in their apical dendrites (arrows). B. GLUT5 staining (green) in cerebellum appears in cells of the Purkinje layer (P) and in their apical dendrites (arrows). Cell nuclei are stained with DAPI (blue).
- Lacombe, V.A. (2014) ISRN Vet. Sci. 2014, 409547.
- Nomura, N. et al. (2014) Nature 526, 397.
The GLUT protein family belongs to the solute carrier 2 family (SLC2) and is a member of the major facilitator superfamily of membrane transporters. The GLUT proteins have a fold consisting of two symmetrical six transmembrane helix (TM) bundle. In addition, the proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini, and a several conserved residues and motifs designated “sugar transporter signatures”1,2.
Fourteen GLUT isoforms have been identified and are divided into 3 different group classes based on sequence similarity and structural and functional characteristics. GLUT 5,7,9 and 11 belong to class ll group1.
The GLUT transporters are uniporters, which facilitate the diffusion of monosaccharides like glucose and fructose across the cell membrane in a concentration-dependent manner. GLUT5 is specific to fructose and is primarily expressed in the small intestine, but lower levels were also detected in the brain, adipose tissue, kidney, testes and skeletal muscle. Several metabolic disorders and cancers are linked to increased GLUT5 expression2.
Anti-GLUT5 (SLC2A5) Antibody (#AGT-025) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 5 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT5 from human, rat, and mouse samples.
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