Name: Anti-GLUT1 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AGT-041-F

Overview

Cat #: AGT-041-F

Alternative Name Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EEFYNQTWNHRYGES, corresponding to amino acid residues 41-55 of rat GLUT1 (Accession P11167). 1^st extracellular loop.

Accession (Uniprot) Number P11167

Gene ID 24778

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – 14/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label Fluorescein isothiocyanate (FITC).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGT-041), and direct flow cytometry (labeled antibody).

Applications: fc

May also work in: ic*, lci*

Direct flow cytometry

Cell surface detection of GLUT1 in human K562 chronic myeloid leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-GLUT1 (extracellular)-FITC Antibody (#AGT-041-F), (5 µg).
Cell surface detection of GLUT1 in mouse TK-1 T-cell lymphoma cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-GLUT1 (extracellular)-FITC Antibody (#AGT-041-F), (5 µg).

References

Olson, A.L. et al. (1996) Annu. Rev. Nutr 16, 235.
Pardridge, W.M. et al. (1990) J. Biol. Chem. 265, 18035.
Klepper, J. et al. (1999) Neurochem. Res. 24, 587.
Leen, W.G. et al. (2014) J. Neurol. 261, 589.
Amann, T. et al. (2011) Mol. Membr. Biol. 28, 182.

Scientific background

Glucose transporter 1 (GLUT1) belongs to the major facilitator superfamily (MFS). It is encoded by SLC2A1, and mediates basal-level cellular uptake of glucose into many tissues. GLUT1 contains 12 membrane-spanning domains with both the amino and carboxyl termini oriented intracellularly. In addition, a single extracellular N-linked glycosylation site is present¹.

GLUT1 is widely expressed, but it is most abundant in fibroblasts, erythrocytes, and endothelial cells with low levels of expression in muscle, liver, and adipose tissue².

Inactivating mutations of GLUT1, resulting in compromised transport activities for glucose, are associated with diseases as a result of lack of energy supply to the brain³. GLUT1 deficiency syndrome (also known as De Vivo syndrome) is characterized by a spectrum of symptoms including early-onset seizures, microcephaly and retarded development⁴. In addition, elevated expression levels of GLUT1 have been observed in several cancer types, identifying GLUT1 as an important prognostic indicator for tumorigenesis⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GLUT1 (extracellular) Antibody (#AGT-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GLUT1 from human, rat, and mouse samples.

Anti-GLUT1 (extracellular)-FITC Antibody (#AGT-041-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

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We ship worldwide within 24 hours of our next business day.

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Anti-GLUT1 (extracellular)-FITC Antibody