Overview
Alternative Name Glycine receptor subunit alpha-3, GLRA3, GlyRα3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)KNKTEAFALEKFYR, corresponding to amino acid residues 355-368 of rat GlyRα3 (Accession P24524). 2nd intracellular loop.
Accession (Uniprot) Number P24524
Gene ID 114516
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2039889.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
- Rat brain membranes (1:200).
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:200).
3-4. Anti-Glycine Receptor α3 Antibody, preincubated with Glycine Receptor α3 Blocking Peptide (BLP-GR003).
Immunohistochemistry
- Rat trigeminal ganglion sections (1:100) (Bae, J.Y. et al. (2016) Brain Struct. Funct. 221, 4601.).
Expression of Glycine Receptor alpha 3 in rat dorsal raphe nucleus (DRN).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GlyRα3 immunoreactivity (green) appears in DRN neurons (arrows). B. Pre-incubation of the antibody with Glycine Receptor α3 Blocking Peptide (BLP-GR003), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Glycine Receptor alpha 3 in rat substantia nigra pars compacta (SNC).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GlyRα3 immunoreactivity (green) appears in outlines of neurons (arrows). B. Pre-incubation of the antibody with Glycine Receptor α3 Blocking Peptide (BLP-GR003), suppressed staining. Cell nuclei are stained with DAPI (blue).
Scientific background
Glycine is a major neurotransmitter in the mammalian central nervous system.1 The glycine receptor is a ligand gated Cl- channel, mediating synaptic inhibition in the spinal cord, retina and regions of the brain.2
The glycine receptor is a pentamer consisting of α and β subunits. To date there are four known α-subunit isoforms (α1-α4) encoded by different genes.2-4
In humans, two alternative splice variants of the glycine receptor α3 have been described: α3L and α3K, where the α3L splice variant bears an additional segment of 15 amino acids within the long cytoplasmic TM3-4 loop. These variants differ in their desensitization kinetics.3
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
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