Overview
Alternative Name Glycine receptor subunit alpha-3, GLRA3, GlyRα3
Accession (Uniprot) Number P24524
Gene ID 114516
For research purposes only, not for human use
Specifications
Applications
Applications
Western blot
- Rat brain membranes (1:200).
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:200).
3-4. Anti-Glycine Receptor α3 Antibody, preincubated with Glycine Receptor α3 Blocking Peptide (BLP-GR003).
Immunohistochemistry
- Rat trigeminal ganglion sections (1:100) (Bae, J.Y. et al. (2016) Brain Struct. Funct. 221, 4601.).
Expression of Glycine Receptor alpha 3 in rat dorsal raphe nucleus (DRN).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GlyRα3 immunoreactivity (green) appears in DRN neurons (arrows). B. Pre-incubation of the antibody with Glycine Receptor α3 Blocking Peptide (BLP-GR003), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Glycine Receptor alpha 3 in rat substantia nigra pars compacta (SNC).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GlyRα3 immunoreactivity (green) appears in outlines of neurons (arrows). B. Pre-incubation of the antibody with Glycine Receptor α3 Blocking Peptide (BLP-GR003), suppressed staining. Cell nuclei are stained with DAPI (blue).
Scientific Background
Scientific Background
Scientific background
Glycine is a major neurotransmitter in the mammalian central nervous system.1 The glycine receptor is a ligand gated Cl- channel, mediating synaptic inhibition in the spinal cord, retina and regions of the brain.2
The glycine receptor is a pentamer consisting of α and β subunits. To date there are four known α-subunit isoforms (α1-α4) encoded by different genes.2-4
In humans, two alternative splice variants of the glycine receptor α3 have been described: α3L and α3K, where the α3L splice variant bears an additional segment of 15 amino acids within the long cytoplasmic TM3-4 loop. These variants differ in their desensitization kinetics.3
Last Update: 25/03/2025
